ArticleHistochemistry and cell biology2005
Comparative spatial and temporal localisation of perlecan, aggrecan and type I, II and IV collagen in the ovine meniscus: an ageing study.
Article in Histochemistry and cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
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Who cites it
70 citing papers in PubMed, 1 synthesis or guideline pooled it, 135 citations in OpenAlex.
- Comparison of Arthroscopic Partial Meniscectomy to Physical Therapy following Degenerative Meniscus Tears: A Systematic Review and Meta-analysis.BioMed research international · 2020Pooled it
- Medial Osteochondral Defect Drives Matrix and Cell Pathology in Compartment-Matched Meniscus.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Why Hoop Tension Matters: A Biomechanical Perspective on Medial Meniscus Posterior Root Tears-A Narrative Review.Bioengineering (Basel, Switzerland) · 2025Review
- The Meniscus: Basic Science and Therapeutic Approaches.Journal of clinical medicine · 2025Review
- Epigenetic dynamics in meniscus cell migration and its zonal dependency in response to inflammatory conditions.APL bioengineering · 2025Article
- siRNA Treatment Enhances Collagen Fiber Formation in Tissue-Engineered Meniscus via Transient Inhibition of Aggrecan Production.Bioengineering (Basel, Switzerland) · 2024Article
- Epigenetic Dynamics in Meniscus Cell Migration and its Zonal Dependency in Response to Inflammatory Conditions: Implications for Regeneration Strategies.bioRxiv : the preprint server for biology · 2024Article
- Degradation of Proteoglycans and Collagen in Equine Meniscal Tissues.International journal of molecular sciences · 2024Article
- Rapid specialization and stiffening of the primitive matrix in developing articular cartilage and meniscus.Acta biomaterialia · 2023Article
- The Glycosaminoglycan Side Chains and Modular Core Proteins of Heparan Sulphate Proteoglycans and the Varied Ways They Provide Tissue Protection by Regulating Physiological Processes and Cellular Behaviour.International journal of molecular sciences · 2023Review
- Suspension bath bioprinting and maturation of anisotropic meniscal constructs.Biofabrication · 2023Article
- Meniscus Matrix Structural and Biomechanical Evaluation: Age-Dependent Properties in a Swine Model.Bioengineering (Basel, Switzerland) · 2022Article
- The transplantation of particulated juvenile allograft cartilage and synovium for the repair of meniscal defect in a lapine model.Journal of orthopaedic translation · 2022Article
- Macromolecular Interactions in Cartilage Extracellular Matrix Vary According to the Cartilage Type and Location.Cartilage · 2021Article
- Impact of perlecan, a core component of basement membrane, on regeneration of cartilaginous tissues.Acta biomaterialia · 2021Review
- Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells.International journal of molecular sciences · 2021Article
- Tissue-specific parameters for the design of ECM-mimetic biomaterials.Acta biomaterialia · 2021Review
- Intrinsic and growth-mediated cell and matrix specialization during murine meniscus tissue assembly.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Perlecan in Pericellular Mechanosensory Cell-Matrix Communication, Extracellular Matrix Stabilisation and Mechanoregulation of Load-Bearing Connective Tissues.International journal of molecular sciences · 2021Review
- Driving Hierarchical Collagen Fiber Formation for Functional Tendon, Ligament, and Meniscus Replacement.Biomaterials · 2021Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This is the first study to immunolocalise perlecan in meniscal tissues and to demonstrate how its localisation varied with ageing relative to aggrecan and type I, II and IV collagen. Perlecan was present in the middle and inner meniscal zones where it was expressed by cells of an oval or rounded morphology. Unlike the other components visualised in this study, perlecan was strongly cell associated and its levels fell significantly with age onset and cell number decline. The peripheral outer meniscal zones displayed very little perlecan staining other than in small blood vessels. Picrosirius red staining viewed under polarised light strongly delineated complex arrangements of slender discrete randomly oriented collagen fibre bundles as well as transverse, thick, strongly oriented, collagen tie bundles in the middle and outer meniscal zones. The collagen fibres demarcated areas of the meniscus which were rich in anionic toluidine blue positive proteoglycans; immunolocalisations confirmed the presence of aggrecan and perlecan. When meniscal sections were examined macroscopically, type II collagen localisation in the inner meniscal zone was readily evident in the 2- to 7-day-old specimens; this became more disperse in the older meniscal specimens. Type I collagen had a widespread distribution in all meniscal zones at all time points. Type IV collagen was strongly associated with blood vessels in the 2- to 7-day-old meniscal specimens but was virtually undetectable at the later time points (>7 month).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.