ArticlePlant molecular biology2005
Essential amino acids of starch synthase IIa differentiate amylopectin structure and starch quality between japonica and indica rice varieties.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
107 citing papers in PubMed, 290 citations in OpenAlex.
- Effect of Allelic Variations inInternational journal of molecular sciences · 2026Article
- Low amylose content in riceBreeding science · 2025Article
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- Unique starch biosynthesis pathways in wild rice revealed by multi-omics analyses.Plant biotechnology journal · 2025Article
- Physiological and compensatory roles of three starch-branching enzymes in different rice organs.Plant molecular biology · 2025Article
- An Integrative Analysis of the Transcriptome and Proteome of Rice Grain Chalkiness Formation Under High Temperature.Plants (Basel, Switzerland) · 2024Article
- Starch phosphorylation regulates starch granule morphological homogeneity in Arabidopsis thaliana.Plant physiology · 2024Article
- Article
- Exploring the impact of key physicochemical properties of rice on taste quality and instant rice processing.Frontiers in plant science · 2024Article
- A model for the reproduction of amylopectin cluster by coordinated actions of starch branching enzyme isoforms.Plant molecular biology · 2023Review
- Loss of function ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Comparison of the physicochemical properties of starches from maize reciprocal F1 hybrids and their parental lines.Food chemistry: X · 2023Article
- ThreeInternational journal of molecular sciences · 2023Article
- Meta-QTL and haplo-pheno analysis reveal superior haplotype combinations associated with low grain chalkiness under high temperature in rice.Frontiers in plant science · 2023Article
- Combined Effects ofFoods (Basel, Switzerland) · 2022Article
- Effect of Heading Date on the Starch Structure and Grain Yield of Rice Lines with Low Gelatinization Temperature.International journal of molecular sciences · 2022Article
- Dynamic Change in Starch Biosynthetic Enzymes Complexes during Grain-Filling Stages in BEIIb Active and Deficient Rice.International journal of molecular sciences · 2022Article
- Combined Effects of Different Alleles ofPlants (Basel, Switzerland) · 2022Article
- The Starch Physicochemical Properties between Superior and Inferior Grains of Japonica Rice under Panicle Nitrogen Fertilizer Determine the Difference in Eating Quality.Foods (Basel, Switzerland) · 2022Article
- The Function of DNA Demethylase Gene ROS1a Null Mutant on Seed Development in Rice (International journal of molecular sciences · 2022Article
47 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four amino acids were variable between the 'active' indica-type and 'inactive' japonica-type soluble starch synthase IIa (SSIIa) of rice plants; Glu-88 and Gly-604 in SSIIa of indica-cultivars IR36 and Kasalath were replaced by Asp-88 and Ser-604, respectively, in both japonica cultivars Nipponbare and Kinmaze SSIIa, whereas Val-737 and Leu-781 in indica SSIIa were replaced by Met-737 in cv. Nipponbare and Phe-781 in cv. Kinmaze SSIIa, respectively. The SSIIa gene fragments shuffling experiments revealed that Val-737 and Leu-781 are essential not only for the optimal SSIIa activity, but also for the capacity to synthesize indica-type amylopectin. Surprisingly, however, a combination of Phe-781 and Gly-604 could restore about 44% of the SSIIa activity provided that Val-737 was conserved. The introduction of the 'active' indica-type SSIIa gene enabled the japonica-type cv. Kinmaze to synthesize indica-type amylopectin. The starch in the transformed japonica rice plants exhibited gelatinization-resistant properties that are characteristic of indica-rice starch. Transformed lines expressing different levels of the IR36 SSIIa protein produced a variety of starches with amylopectin chain-length distribution patterns that correlated well with their onset temperatures of gelatinization. The present study confirmed that the SSIIa activity determines the type of amylopectin structure of rice starch to be either the typical indica-type or japonica-type, by playing a specific role in the synthesis of the long B(1) chains by elongating short A and B(1) chains, notwithstanding the presence of functional two additional SSII genes, a single SSI gene, two SSIII genes, and two SSIV genes in rice plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.