ArticleMolecular and cellular biology2005
Components of the ESCRT pathway, DFG16, and YGR122w are required for Rim101 to act as a corepressor with Nrg1 at the negative regulatory element of the DIT1 gene of Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 80 citations in OpenAlex.
- Rsp5/NEDD4 and ESCRT regulate TDP-43 toxicity and turnover via an endolysosomal clearance mechanism.The Journal of cell biology · 2026Article
- Mechanism of enhanced salt tolerance in Saccharomyces cerevisiae by CRZ1 overexpression.Scientific reports · 2024Article
- Conservation of the Pal/Rim Pathway in Ustilaginomycetes.Current microbiology · 2024Article
- Conserved and Divergent Features of pH Sensing in Major Fungal Pathogens.Current clinical microbiology reports · 2023Review
- Interactions in the ESCRT-III network of the yeast Saccharomyces cerevisiae.Current genetics · 2019Article
- Aggregate Filamentous Growth Responses in Yeast.mSphere · 2019Article
- Characterization of additional components of the environmental pH-sensing complex in the pathogenic fungusThe Journal of biological chemistry · 2018Article
- Involvement of the exomer complex in the polarized transport of Ena1 required forMolecular biology of the cell · 2017Article
- Mutational analysis of the Aspergillus ambient pH receptor PalH underscores its potential as a target for antifungal compounds.Molecular microbiology · 2016Article
- Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein.Molecular microbiology · 2015Article
- Casein kinase 1 controls the activation threshold of an α-arrestin by multisite phosphorylation of the interdomain hinge.Molecular biology of the cell · 2015Article
- Causal variation in yeast sporulation tends to reside in a pathway bottleneck.PLoS genetics · 2014Article
- pH signaling in human fungal pathogens: a new target for antifungal strategies.Eukaryotic cell · 2014Review
- The Cryptococcus neoformans Rim101 transcription factor directly regulates genes required for adaptation to the host.Molecular and cellular biology · 2014Article
- Interaction maps of the Saccharomyces cerevisiae ESCRT-III protein Snf7.Eukaryotic cell · 2013Article
- Variance heterogeneity in Saccharomyces cerevisiae expression data: trans-regulation and epistasis.PloS one · 2013Article
- cis- and trans-acting localization determinants of pH response regulator Rim13 in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- pH Response Pathways in Fungi: Adapting to Host-derived and Environmental Signals.Mycobiology · 2011Article
- Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling.Journal of cell science · 2011Article
- Sporulation in the budding yeast Saccharomyces cerevisiae.Genetics · 2011Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The divergently transcribed DIT1 and DIT2 genes of Saccharomyces cerevisiae, which belong to the mid-late class of sporulation-specific genes, are subject to Ssn6-Tup1-mediated repression in mitotic cells. The Ssn6-Tup1 complex, which is required for repression of diverse sets of coordinately regulated genes, is known to be recruited to target genes by promoter-specific DNA-binding proteins. In this study, we show that a 42-bp negative regulatory element (NRE) present in the DIT1-DIT2 intergenic region consists of two distinct subsites and that a multimer of each subsite supports efficient Ssn6-Tup1-dependent repression of a CYC1-lacZ reporter gene. By genetic screening procedures, we identified DFG16, YGR122w, VPS36, and the DNA-binding proteins Rim101 and Nrg1 as potential mediators of NRE-directed repression. We show that Nrg1 and Rim101 bind simultaneously to adjacent target sites within the NRE in vitro and act as corepressors in vivo. We have found that the ability of Rim101 to be proteolytically processed to its active form and mediate NRE-directed repression not only depends on the previously characterized RIM signaling pathway but also requires Dfg16, Ygr122w, and components of the ESCRT trafficking pathway. Interestingly, Rim101 was processed in bro1 and doa4 strains but was unable to mediate efficient repression.
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