ArticlePlant physiology2005
Functional analysis of the pyrimidine de novo synthesis pathway in solanaceous species.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Strategic global data integration to improve genomic prediction accuracy in tree breeding programs facing resource limitations, a case study in mango.Horticulture research · 2026Article
- Topological criterion for robust perfect adaptation of reaction fluxes in biological networks.iScience · 2025Article
- Review of the novel antifungal drug olorofim (F901318).BMC infectious diseases · 2024Review
- A Novel mechanism of herbicide action through disruption of pyrimidine biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings.Frontiers in plant science · 2023Article
- Genome and transcriptome-wide study of carbamoyltransferase genes in major fleshy fruits: A multi-omics study of evolution and functional significance.Frontiers in plant science · 2022Article
- Mechanisms of feedback inhibition and sequential firing of active sites in plant aspartate transcarbamoylase.Nature communications · 2021Article
- Overexpression of Phosphoribosyl Pyrophosphate Synthase Enhances Resistance ofFrontiers in plant science · 2021Article
- CTP Synthase 2 FromFrontiers in plant science · 2021Article
- SNPeffect: identifying functional roles of SNPs using metabolic networks.The Plant journal : for cell and molecular biology · 2020Article
- Advances in understanding salt tolerance in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis.Scientific reports · 2019Article
- Article
- Effect of the Novel Antifungal Drug F901318 (Olorofim) on Growth and Viability of Aspergillus fumigatus.Antimicrobial agents and chemotherapy · 2018Article
- Nucleobase and nucleoside transport and integration into plant metabolism.Frontiers in plant science · 2014Review
- Early gene expression events in the laminar abscission zone of abscission-promoted citrus leaves after a cycle of water stress/rehydration: involvement of CitbHLH1.Journal of experimental botany · 2012Article
- Inhibition of p-aminobenzoate and folate syntheses in plants and apicomplexan parasites by natural product rubreserine.The Journal of biological chemistry · 2012Article
- Article
- Arabidopsis thaliana nucleosidase mutants provide new insights into nucleoside degradation.The New phytologist · 2011Article
- Profiles of the biosynthesis and metabolism of pyridine nucleotides in potatoes (Solanum tuberosum L.).Planta · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrimidines are particularly important in dividing tissues as building blocks for nucleic acids, but they are equally important for many biochemical processes, including sucrose and cell wall polysaccharide metabolism. In recent years, the molecular organization of nucleotide biosynthesis in plants has been analyzed. Here, we present a functional analysis of the pyrimidine de novo synthesis pathway. Each step in the pathway was investigated using transgenic plants with reduced expression of the corresponding gene to identify controlling steps and gain insights into the phenotypic and metabolic consequences. Inhibition of expression of 80% based on steady-state mRNA level did not lead to visible phenotypes. Stepwise reduction of protein abundance of Asp transcarbamoylase or dihydro orotase resulted in a corresponding inhibition of growth. This was not accompanied by pleiotropic effects or by changes in the developmental program. A more detailed metabolite analysis revealed slightly different responses in roots and shoots of plants with decreased abundance of proteins involved in pyrimidine de novo synthesis. Whereas in leaves the nucleotide and amino acid levels were changed only in the very strong inhibited plants, the roots show a transient increase of these metabolites in intermediate plants followed by a decrease in the strong inhibited plants. Growth analysis revealed that elongation rates and number of organs per plant were reduced, without large changes in the average cell size. It is concluded that reduced pyrimidine de novo synthesis is compensated for by reduction in growth rates, and the remaining nucleotide pools are sufficient for running basic metabolic processes.
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