ArticlePlant physiology2005
Characterization of vacuolar transport of the endogenous alkaloid berberine in Coptis japonica.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 123 citations in OpenAlex.
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- Role of ABC Proteins in Secondary Metabolism and Immune (=Defensive) Response in Seaweeds.Cells · 2023Article
- Metabolomics analysis reveals the accumulation patterns of flavonoids and phenolic acids in quinoa (Food chemistry: X · 2023Article
- Cell type matters: competence for alkaloid metabolism differs in two seed-derived cell strains of Catharanthus roseus.Protoplasma · 2023Article
- Novel Cognitions in Allelopathy: Implications from the "Horizontal Natural Product Transfer".Plants (Basel, Switzerland) · 2022Review
- Characterization of Triterpene Saponin Glycyrrhizin Transport byPlants (Basel, Switzerland) · 2022Article
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- Plant tissue culture as a perpetual source for production of industrially important bioactive compounds.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Modulatory Effect ofMolecules (Basel, Switzerland) · 2020Article
- Skin transcriptional profiles in Oophaga poison frogs.Genetics and molecular biology · 2020Article
- Proton Gradient-Dependent Transport of p-Glucocoumaryl Alcohol in Differentiating Xylem of Woody Plants.Scientific reports · 2019Article
- Inhibitory Effect of Berberine on Broiler P-glycoprotein Expression and Function: In Situ and In Vitro Studies.International journal of molecular sciences · 2019Article
- Comparison of Strategies to Overcome Drug Resistance: Learning from Various Kingdoms.Molecules (Basel, Switzerland) · 2018Review
- Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus.Horticulture research · 2018Article
- The NorM MATE transporter from N. gonorrhoeae: insights into drug and ion binding from atomistic molecular dynamics simulations.Biophysical journal · 2014Article
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- Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells.Frontiers in plant science · 2013Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alkaloids comprise one of the largest groups of plant secondary metabolites. Many of them exhibit strong biological activities, and, in most cases, they are accumulated in the central vacuole of alkaloid-producing plants after synthesis. However, the mechanisms involved in alkaloid transport across the tonoplast are only poorly understood. In this study, we analyzed the vacuolar transport mechanism of an isoquinoline alkaloid, berberine, which is produced and accumulated in the vacuole of cultured cells of Coptis japonica. The characterization of berberine transport using intact vacuoles and a tonoplast vesicle system showed that berberine uptake was stimulated by Mg/ATP, as well as GTP, CTP, UTP, and Mg/pyrophosphate. Berberine uptake was strongly inhibited by NH4(+) and bafilomycin A1, while vanadate, which is commonly used to inhibit ATP-binding cassette transporters, had only a slight effect, which suggests the presence of a typical secondary transport mechanism. This is contrary to the situation in the plasma membrane of this plant cell, where the ATP-binding cassette transporter is involved in berberine transport. Model experiments with liposomes demonstrated that an ion-trap mechanism was hardly implicated in berberine transport. Further studies suggested that berberine was transported across the tonoplast via an H+/berberine antiporter, which has a Km value of 43.7 microM for berberine. Competition experiments using various berberine analogs, as well as other classes of alkaloids, revealed that this transporter is fairly specific, but not exclusive, for berberine.
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