ArticleGenes & development2005
A Rik1-associated, cullin-dependent E3 ubiquitin ligase is essential for heterochromatin formation.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
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Who cites it
123 citing papers in PubMed, 192 citations in OpenAlex.
- Substrate recognition, not sequestration, drives the engagement of an H3K9 methyltransferase in living cells.bioRxiv : the preprint server for biology · 2026Article
- Nucleosome remodeling by a CHD enzyme promotes H3K9 methylation establishment and spreading via remodeler-writer feedback.bioRxiv : the preprint server for biology · 2026Article
- Article
- An H3K14ub-H3K9me3 feedback circuit governs heterochromatin spreading and inheritance in fission yeast.Nature communications · 2026Article
- Article
- Transcriptional PBR cycles at pericentromeric repeats cause gross chromosomal rearrangements through Rad52-dependent ADR-loop formation.Nucleic acids research · 2026Article
- Article
- Intrinsically disordered region of Clr4/Suv39 regulates its enzymatic activity and ensures heterochromatin spreading.Nucleic acids research · 2025Article
- Mechanistic insights into the stimulation of the histone H3K9 methyltransferase Clr4 by proximal H3K14 ubiquitination.Science advances · 2025Article
- Fission yeast Caprin protein is required for efficient heterochromatin establishment.PLoS genetics · 2025Article
- Review
- A systematic quantitative approach comprehensively defines domain-specific functional pathways linked to Schizosaccharomyces pombe heterochromatin regulation.Nucleic acids research · 2024Article
- Clr4Nature communications · 2024Article
- Heterochromatin repeat organization at an individual level: Rex1BD and the 14-3-3 protein coordinate to shape the epigenetic landscape within heterochromatin repeats.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.Cell · 2024Article
- The SUN-family protein Sad1 mediates heterochromatin spatial organization through interaction with histone H2A-H2B.Nature communications · 2024Article
- A systematic quantitative approach comprehensively defines domain-specific functional pathways linked tobioRxiv : the preprint server for biology · 2024Article
- Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Cross-Regulation Between Set1, Clr4, and Lsd1/2 in Schizosaccharomyces pombe.PLoS genetics · 2024Article
- Rex1BD and the 14-3-3 protein control heterochromatin organization at tandem repeats by linking RNAi and HDAC.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
63 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Heterochromatin is critical for proper centromere and telomere function, and it plays a key role in the transcriptional silencing of specific genomic loci. In fission yeast, the Rik1 protein functions with the Clr4 histone methyltransferase at an early step in heterochromatin formation. Here, we use mass spectrometry and tandem affinity purification of a Rik1-TAP fusion protein to identify Rik1-associated proteins. These studies identify two novel proteins, Raf1 and Raf2, which we find are required for H3-K9 methylation and for transcriptional silencing within centromeric heterochromatin. We also find that subunits of a cullin-dependent E3 ubiquitin ligase are associated with Rik1 and Clr4, and Rik1-TAP preparations exhibit robust E3 ubiquitin ligase activity. Furthermore, expression of a dominant-negative allele of the Pcu4 cullin subunit disrupts regulation of K4 methylation within heterochromatin. These studies provide evidence for a novel Rik1-associated E3 ubiquitin ligase that is required for heterochromatin formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.