ArticleJournal of molecular biology2005
Tissue-specific regulation of the mouse alphaA-crystallin gene in lens via recruitment of Pax6 and c-Maf to its promoter.
Article in Journal of molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 61 citations in OpenAlex.
- RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.Developmental biology · 2026Article
- Analysis of long-range chromatin contacts, compartments and looping between mouse embryonic stem cells, lens epithelium and lens fibers.Epigenetics & chromatin · 2024Article
- Dynamic changes in whole genome DNA methylation, chromatin and gene expression during mouse lens differentiation.Epigenetics & chromatin · 2023Article
- Expression of Maf family proteins in glutamatergic neurons of the mouse olfactory bulb.Developmental neurobiology · 2022Article
- Crystallin gene expression: Insights from studies of transcriptional bursting.Experimental eye research · 2021Review
- Mutation update of transcription factor genes FOXE3, HSF4, MAF, and PITX3 causing cataracts and other developmental ocular defects.Human mutation · 2018Review
- N-myc regulates growth and fiber cell differentiation in lens development.Developmental biology · 2017Article
- Evolutionary Origins of Pax6 Control of Crystallin Genes.Genome biology and evolution · 2017Article
- Article
- Chromatin remodeling enzyme Snf2h regulates embryonic lens differentiation and denucleation.Development (Cambridge, England) · 2016Article
- Regulation of c-Maf and αA-Crystallin in Ocular Lens by Fibroblast Growth Factor Signaling.The Journal of biological chemistry · 2016Article
- Pax6 associates with H3K4-specific histone methyltransferases Mll1, Mll2, and Set1a and regulates H3K4 methylation at promoters and enhancers.Epigenetics & chromatin · 2016Article
- A conserved role of αA-crystallin in the development of the zebrafish embryonic lens.Experimental eye research · 2015Article
- Identification of in vivo DNA-binding mechanisms of Pax6 and reconstruction of Pax6-dependent gene regulatory networks during forebrain and lens development.Nucleic acids research · 2015Article
- Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.Cell · 2015Article
- Chromatin features, RNA polymerase II and the comparative expression of lens genes encoding crystallins, transcription factors, and autophagy mediators.Molecular vision · 2015Article
- Differential effect of cataract-associated mutations in MAF on transactivation of MAF target genes.Molecular and cellular biochemistry · 2014Article
- Pax6-dependent, but β-catenin-independent, function of Bcl9 proteins in mouse lens development.Genes & development · 2014Article
- The role of a lens survival pathway including sox2 and αA-crystallin in the evolution of cavefish eye degeneration.EvoDevo · 2014Article
- Identification and characterization of FGF2-dependent mRNA: microRNA networks during lens fiber cell differentiation.G3 (Bethesda, Md.) · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Pax6 is a lineage-restricted DNA-binding transcription factor regulating the formation of mammalian organs including brain, eye and pancreas. Pax6 plays key roles during the initial formation of lens lineage, proliferation of lens progenitor and precursor cells and their terminal differentiation. In addition to Pax6, lens fiber cell differentiation is regulated by c-Maf, Prox1 and Sox1. Crystallins are essential lens structural proteins required for light refraction and transparency. Mouse alphaA-crystallin represents about 17% of all crystallins at the protein level and ranks as one of the most abundant tissue-specific proteins. Lens-specific expression of this gene is regulated at the level of transcription. A promoter fragment of -88 to +46 is capable of driving lens-specific expression in transgenic mouse. Here we provide data suggesting that this lens-specific promoter fragment is comprised of multiple Pax6 and Maf-binding sites. Site-directed mutagenesis of regions within these sites resulted in partially or completely reduced promoter activities in lens cells. Co-transfections using Pax6 and c-Maf alone revealed moderate and strong activations of this promoter, respectively. In contrast to synergistic activation of alphaB-crystallin by Pax6 and c-Maf, Pax6 has a neutral effect on c-Maf-mediated alphaA-crystallin promoter activation. Chromatin immunoprecipitations established in vivo interactions of Pax6 and c-Maf with the alphaA-crystallin promoter in lens cells. Collectively, the present data support a molecular model in which tissue-specific expression of alphaA-crystallin is regulated by recruitment of Pax6 and c-Maf, two proteins regulating multiple processes of lens differentiation, to its promoter. In addition, the data suggest a molecular model of temporal and spatial regulation of alphaB, alphaA and gamma-crystallin genes in mouse embryonic lens by using variants of the Pax6/Maf regulatory module.
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