ArticleTransgenic research2005
Production of biologically active GM-CSF in sugarcane: a secure biofactory.
Article in Transgenic research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 84 citations in OpenAlex.
- Increasing the Efficiency of the Accumulation of Recombinant Proteins in Plant Cells: The Role of Transport Signal Peptides.Plants (Basel, Switzerland) · 2022Review
- Improving Protein Quantity and Quality-The Next Level of Plant Molecular Farming.International journal of molecular sciences · 2022Review
- Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system.Scientific reports · 2020Article
- High-Level Production of Recombinant Snowdrop Lectin in Sugarcane and Energy Cane.Frontiers in bioengineering and biotechnology · 2020Article
- In planta production and characterization of a hyperthermostable GH10 xylanase in transgenic sugarcane.Plant molecular biology · 2017Article
- Vacuolar targeting of r-proteins in sugarcane leads to higher levels of purifiable commercially equivalent recombinant proteins in cane juice.Plant biotechnology journal · 2016Article
- Isolation and Characterization of ScGluD2, a New Sugarcane beta-1,3-Glucanase D Family Gene Induced by Sporisorium scitamineum, ABA, H2O2, NaCl, and CdCl2 Stresses.Frontiers in plant science · 2016Article
- Plant Based Bioreactors of Recombinant Cytokines (Review).Applied biochemistry and microbiology · 2016Review
- Tissue-specific transcriptome analysis within the maturing sugarcane stalk reveals spatial regulation in the expression of cellulose synthase and sucrose transporter gene families.Plant molecular biology · 2015Article
- Improved molecular tools for sugar cane biotechnology.Plant molecular biology · 2014Article
- Tobacco as biofactory for biologically active hPL production: a human hormone with potential applications in type-1 diabetes.Transgenic research · 2011Article
- Embryogenic callus proliferation and regeneration conditions for genetic transformation of diverse sugarcane cultivars.Plant cell reports · 2011Article
- Recombinant cytokines from plants.International journal of molecular sciences · 2011Review
- Plant-made vaccine antigens and biopharmaceuticals.Trends in plant science · 2009Review
- Biologically active human GM-CSF produced in the seeds of transgenic rice plants.Transgenic research · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over 300 transgenic sugarcane plants representing approx. 200 independent lines producing the human cytokine granulocyte macrophage colony stimulating factor (GM-CSF) were analyzed for recombinant protein accumulation and activity levels. Expression constructs differed in use of the maize polyubiquitin 1, Mubi-1, or the sugarcane polyubiquitin 9, SCubi9, promoters; presence or absence of a C-terminal HDEL tag for ER retention; and presence or absence of a 6X Histidine tag for metal ion affinity purification. Accumulation of GM-CSF protein ranged from undetectable to 0.02% of total soluble protein. No significant difference was observed between the two promoters; however, the ER retention tag was required for higher accumulation levels. Human bone marrow cells (TF-1), which require GM-CSF for cell division, proliferated when growth media was supplemented with transgenic sugarcane extracts. Comparison to purified commercially produced GM-CSF indicated the sugarcane-produced protein had essentially identical activity levels. In a 14-month field trial, accumulation levels remained stable. This is the first report of field production of GM-CSF. During the field trial, no flowering of the trial plants occurred; no pollen or seed was produced. Drying, burning, and burial of the test plants effectively blocked possible routes for the transgenic sugarcane to enter the environment or food supply. Sugarcane may provide a highly secure system for biofactory production of pharmaceutical proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.