ArticleMolecular biology reports2005
Isolation and characterization of simple repeat sequences from the yellow fin sea bream Acanthopagrus latus (Sparidae).
Article in Molecular biology reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 7 citations in OpenAlex.
- Genetic variation and phylogenetic relationship between two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris (Teleostei: Horabagridae) based on RAPD and microsatellite markers.Molecular biology reports · 2011Article
- A target site for spontaneous insertion of IS10 element in pUC19 DNA located within intrinsically bent DNA.The open microbiology journal · 2009Article
- Isolation of sperm vesicles from adult male mayflies and other insects to prepare high molecular weight genomic DNA samples.Molecular biology reports · 2006Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We isolated DNA fragments containing various repetitive elements from the genome of a sea bream Acanthopagrus latus. Sequence analysis indicated that two fragments have particularly interesting features. Fragment AL87 contained a tetranucleotide repeat and a quasipalindromic sequence. Sequence comparison suggested that AL87 may be a part of a gene encoding a serine/threonine protein kinase, and that the quasipalindrome is situated at the junction of an intron and an exon. Moreover, the quasipalindrome is conserved in several other fishes, even though it has the potential to form a stem-loop structure at the splicing site. Fragment AL79 contained a minisatellite sequence made up of six 30-bp units in tandem. DNase I sensitivity assays and statistical analyses showed the repeat region to be flexible when subjected to bending stress. In addition, atomic force microscopic imaging of AL79 showed the presence of highly curved (kinked) segments flanking the repeat region. The structural features of these repetitive elements may be key factors facilitating the amplification of the repeats.
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