ArticlePlant molecular biology2005
Identification of differentially expressed genes in leaf and root between wheat hybrid and its parental inbreds using PCR-based cDNA subtraction.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Transcriptome dynamics and allele-specific regulation underlie wheat heterosis at the anthesis and grain-filling stages.BMC genomics · 2025Article
- Genome‑wide identification and expression analysis of the UBC gene family in wheat (Triticum aestivum L.).BMC plant biology · 2024Article
- Research on Physiological Characteristics and Differential Gene Expression of Rice Hybrids and Their Parents under Salt Stress at Seedling Stage.Plants (Basel, Switzerland) · 2024Article
- Advances in Research on the Mechanism of Heterosis in Plants.Frontiers in plant science · 2021Review
- Comparative transcriptome analysis between inbred and hybrids reveals molecular insights into yield heterosis of upland cotton.BMC plant biology · 2020Article
- Differential Expression of Genes at Panicle Initiation and Grain Filling Stages Implied in Heterosis of Rice Hybrids.International journal of molecular sciences · 2020Article
- An ex vivo ruminal ovine model to study the immediate immune response in the context of bacterial lipopolysaccharide.Functional & integrative genomics · 2018Article
- Comparative Transcriptome Analysis Reveals Growth-Related Genes in Juvenile Chinese Sea Cucumber, Russian Sea Cucumber, and Their Hybrids.Marine biotechnology (New York, N.Y.) · 2018Article
- Nitrogen Use Efficiency and Carbon Traits of High-Yielding European Hybrid vs. Line Winter Wheat Cultivars: Potentials and Limitations.Frontiers in plant science · 2018Article
- Identification and Expression Analysis of WheatFrontiers in genetics · 2018Article
- Transcriptome analysis of genes involved in defence response in Polyporus umbellatus with Armillaria mellea infection.Scientific reports · 2015Article
- Comparative transcriptional profiling of three super-hybrid rice combinations.International journal of molecular sciences · 2014Article
- Identification of candidate genes associated with positive and negative heterosis in rice.PloS one · 2014Article
- Transcriptome analysis of rice root heterosis by RNA-Seq.BMC genomics · 2013Article
- Comparative proteomic analysis of embryos between a maize hybrid and its parental lines during early stages of seed germination.PloS one · 2013Article
- Transcriptome analysis of artificial hybrid pufferfish Jiyan-1 and its parental species: implications for pufferfish heterosis.PloS one · 2013Article
- Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice.Molecular plant · 2010Article
- A transcriptomic analysis of superhybrid rice LYP9 and its parents.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- HRGD: a database for mining potential heterosis-related genes in plants.Plant molecular biology · 2009Article
- Identification of differentially expressed proteins between hybrid and parents in wheat (Triticum aestivum L.) seedling leaves.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterosis was defined as the advantage of hybrid performance over its parents in terms of growth and productivity. Previous studies showed that differential gene expression between hybrids and their parents is responsible for the heterosis; however, information on systematic identification and characterization of the differentially expressed genes are limited. In this study, an interspecific hybrid between common wheat (Triticum aestivum. L., 2n = 6x = 42, AABBDD) line 3338 and spelt (Triticum spelta L. 2n = 6x = 42, AABBDD) line 2463 was found to be highly heterotic in both aerial growth and root related traits, and was then used for expression assay. A modified suppression subtractive hybridization (SSH) was used to generate four subtracted cDNA libraries, and 748 nonreduandant cDNAs were obtained, among which 465 had high sequence similarity to the GenBank entries and represent diverse of functional categories, such as metabolism, cell growth and maintenance, signal transduction, photosynthesis, response to stress, transcription regulation and others. The expression patterns of 68.2% SSH-derived cDNAs were confirmed by reverse Northern blot, and semi-quantitative RT-PCR exhibited the similar results (72.2%). And it was concluded that the genes differentially expressed between hybrids and their parents involved in diverse physiological process pathway, which might be responsible for the observed heterosis.
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