ArticleGenetics2005
Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 35 citations in OpenAlex.
- RebL1 is required for macronuclear structure stability and gametogenesis inMarine life science & technology · 2024Article
- The Role of Chromatin Assembly Factors in Induced Mutagenesis at Low Levels of DNA Damage.Genes · 2023Article
- Comprehensive characterization of the Hsp70 interactome reveals novel client proteins and interactions mediated by posttranslational modifications.PLoS biology · 2022Article
- Distinct associations of the Saccharomyces cerevisiae Rad9 protein link Mac1-regulated transcription to DNA repair.Current genetics · 2020Article
- A novel method of using Deep Belief Networks and genetic perturbation data to search for yeast signaling pathways.PloS one · 2018Article
- The Candida albicans HIR histone chaperone regulates the yeast-to-hyphae transition by controlling the sensitivity to morphogenesis signals.Scientific reports · 2017Article
- The Ddc1-Mec3-Rad17 sliding clamp regulates histone-histone chaperone interactions and DNA replication-coupled nucleosome assembly in budding yeast.The Journal of biological chemistry · 2014Article
- Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity.SpringerPlus · 2014Article
- Genome-wide single-cell-level screen for protein abundance and localization changes in response to DNA damage in S. cerevisiae.Nucleic acids research · 2013Article
- Differential analysis of high-throughput quantitative genetic interaction data.Genome biology · 2012Article
- Review
- Surprising complexity of the Asf1 histone chaperone-Rad53 kinase interaction.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A barcode screen for epigenetic regulators reveals a role for the NuB4/HAT-B histone acetyltransferase complex in histone turnover.PLoS genetics · 2011Article
- ASF1A and ATM regulate H3K56-mediated cell-cycle checkpoint recovery in response to UV irradiation.Nucleic acids research · 2011Article
- Patterns and mechanisms of ancestral histone protein inheritance in budding yeast.PLoS biology · 2011Article
- A common telomeric gene silencing assay is affected by nucleotide metabolism.Molecular cell · 2011Article
- Transcriptional regulation by Asf1: new mechanistic insights from studies of the DNA damage response to replication stress.The Journal of biological chemistry · 2011Article
- Perspectives on the DNA damage and replication checkpoint responses in Saccharomyces cerevisiae.DNA repair · 2009Review
- HPC2 and ubinuclein define a novel family of histone chaperones conserved throughout eukaryotes.Molecular bioSystems · 2009Article
- Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
CAF-1, Hir proteins, and Asf1 are histone H3/H4 binding proteins important for chromatin-mediated transcriptional silencing. We explored genetic and physical interactions between these proteins and S-phase/DNA damage checkpoint kinases in the budding yeast Saccharomyces cerevisiae. Although cells lacking checkpoint kinase Mec1 do not display defects in telomeric gene silencing, silencing was dramatically reduced in cells lacking both Mec1 and the Cac1 subunit of CAF-1. Silencing was restored in cac1Delta and cac1Delta mec1Delta cells upon deletion of Rad53, the kinase downstream of Mec1. Restoration of silencing to cac1Delta cells required both Hir1 and Asf1, suggesting that Mec1 counteracts functional sequestration of the Asf1/Hir1 complex by Rad53. Consistent with this idea, the degree of suppression of silencing defects by rad53 alleles correlated with effects on Asf1 binding. Furthermore, deletion of the Dun1 kinase, a downstream target of Rad53, also suppressed the silencing defects of cac1Delta cells and reduced the levels of Asf1 associated with Rad53 in vivo. Loss of Mec1 and Rad53 did not alter telomere lengths or Asf1 protein levels, nuclear localization, or chromosome association. We conclude that the Mec1 and Dun1 checkpoint kinases regulate the Asf1-Rad53 interaction and therefore affect the activity of the Asf1/Hir complex in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.