ArticleThe Journal of physiology2005
Electrophysiological and molecular identification of voltage-gated sodium channels in murine vascular myocytes.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 73 citations in OpenAlex.
- Shared transcriptional regulators and network rewiring identify therapeutic targets linking type 2 diabetes mellitus and hypertension.Frontiers in molecular biosciences · 2025Article
- Safinamide, an inhibitor of monoamine oxidase, modulates the magnitude, gating, and hysteresis of sodium ion current.BMC pharmacology & toxicology · 2024Article
- Modulation of fast sodium current in airway smooth muscle cells by exchange protein directly activated by cAMP.American journal of physiology. Cell physiology · 2024Article
- Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques.Frontiers in physiology · 2023Review
- Effective Perturbations by Small-Molecule Modulators on Voltage-Dependent Hysteresis of Transmembrane Ionic Currents.International journal of molecular sciences · 2022Review
- Characterization of Direct Perturbations on Voltage-Gated Sodium Current by Esaxerenone, a Nonsteroidal Mineralocorticoid Receptor Blocker.Biomedicines · 2021Article
- Inhibition of overexpressed Kv3.4 augments HPV in endotoxemic mice.BMC pulmonary medicine · 2020Article
- Elucidation of Cellular Mechanisms That Regulate the Sustained Contraction and Relaxation of the Mammalian Iris.Investigative ophthalmology & visual science · 2020Article
- Veratridine modifies the gating of human voltage-gated sodium channel Nav1.7.Acta pharmacologica Sinica · 2018Article
- Axonal sodium channel NaV1.2 drives granule cell dendritic GABA release and rapid odor discrimination.PLoS biology · 2018Article
- Effects of 4,9-anhydrotetrodotoxin on voltage-gated NaNaunyn-Schmiedeberg's archives of pharmacology · 2018Article
- Voltage-dependent inward currents in smooth muscle cells of skeletal muscle arterioles.PloS one · 2018Article
- Sodium channel Nav1.7 in vascular myocytes, endothelium, and innervating axons in human skin.Molecular pain · 2015Article
- Article
- Cardiac ion channels.Channels (Austin, Tex.) · 2015Review
- Electrophysiological Differences between the Same Pore Region Mutation in SCN1A and SCN3A.Molecular neurobiology · 2015Article
- The effect of high [K(+)]o on spontaneous Ca(2+) waves in freshly isolated interstitial cells of Cajal from the rabbit urethra.Physiological reports · 2014Article
- Effects of age on expression of BKca channel in vascular smooth muscle cells from mesenteric arteries of spontaneously hypertensive rats.Journal of physiology and biochemistry · 2013Article
- Maximum shortening velocity of lymphatic muscle approaches that of striated muscle.American journal of physiology. Heart and circulatory physiology · 2013Article
- Channelopathy pathogenesis in autism spectrum disorders.Frontiers in genetics · 2013Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A voltage-gated Na+ current was characterised in freshly dissociated mouse portal vein (PV) smooth muscle myocytes. The current was found superimposed upon the relatively slow L-type Ca2+ current and was resistant to conventional Ca2+ channel blockers but was abolished by external Na+ replacement and tetrodotoxin (TTX, 1 microM). The molecular identity of the channel responsible for this conductance was determined by RT-PCR where only the transcripts for Na+ channel genes SCN7a, 8a and 9a were detected. The presence of the protein counterparts to the SCN8a and 9a genes (NaV1.6 and NaV1.7, respectively) on the individual smooth muscle myocytes were confirmed in immunocytochemistry, which showed diffuse staining around a predominantly plasmalemmal location. TTX inhibited the action potential in individual myocytes generated in the current clamp mode but isometric tissue tension experiments revealed that TTX (1 and 5 microM) had no effect on the inherent mouse PV rhythmicity. However, the Na+ channel opener veratridine (10 and 50 microM) significantly increased the length of contraction and the interval between contractions. This effect was not influenced by pre-incubation with atropine, prazosin and propranolol, but was reversed by TTX (1 microM) and completely abolished by nicardipine (1 microM). Furthermore, preincubation with the reverse-mode Na+-Ca2+ exchange blocker KB-R7943 (10 microM) also inhibited the veratridine response. We have established for the first time the molecular identity of the voltage-gated Na+ channel in freshly dispersed smooth muscle cells and have shown that these channels can modulate contractility through a novel mechanism of action possibly involving reverse mode Na+-Ca2+ exchange.
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