ArticleThe Journal of physiology2005
TASK-like K+ channels mediate effects of 5-HT and extracellular pH in rat dorsal vagal neurones in vitro.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 27 citations in OpenAlex.
- Blockade of Na+/H+ exchanger type 3 causes intracellular acidification and hyperexcitability via inhibition of pH-sensitive K+ channels in chemosensitive respiratory neurons of the dorsal vagal nucleus in rats.Neuroscience bulletin · 2014Article
- Immunocytochemical localization of TASK-3 protein (K2P9.1) in the rat brain.Cellular and molecular neurobiology · 2014Article
- Cardioprotection evoked by remote ischaemic preconditioning is critically dependent on the activity of vagal pre-ganglionic neurones.Cardiovascular research · 2012Article
- Essential role of Phox2b-expressing ventrolateral brainstem neurons in the chemosensory control of inspiration and expiration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Protein kinase G dynamically modulates TASK1-mediated leak K+ currents in cholinergic neurons of the basal forebrain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Silencing of ventromedial hypothalamic neurons by glucose-stimulated K(+) currents.Pflugers Archiv : European journal of physiology · 2009Article
- Serotonin increases GABA release in rat entorhinal cortex by inhibiting interneuron TASK-3 K+ channels.Molecular and cellular neurosciences · 2008Article
- Mitochondrial expression of the two-pore domain TASK-3 channels in malignantly transformed and non-malignant human cells.Virchows Archiv : an international journal of pathology · 2008Article
- Control of hypothalamic orexin neurons by acid and CO2.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Ionic currents underlying the response of rat dorsal vagal neurones to hypoglycaemia and chemical anoxia.The Journal of physiology · 2007Article
- Neuronal two-pore-domain potassium channels and their regulation by G protein-coupled receptors.The Journal of physiology · 2007Review
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Dorsal vagal neurones (DVN) receive serotonergic projections from the medullary raphé nuclei, suggesting that 5-HT modulates vagal activity. A previous study has shown that 5-HT excites DVN in part by inhibition of a K+ current via postsynaptic 5-HT2A receptors. As mRNA for the two-pore-domain K+ channels TASK-1 (KCNK3) and TASK-3 (KCNK9) has been found in DVN, we investigated the possibility that 5-HT exerts its effects via inhibition of these K+ channels using whole-cell patch-clamp techniques. In current clamp, 5-HT (20 microM) elicited a depolarization by 5.1+/-1.5 mV and an increase in firing rate. In voltage clamp, 5-HT reduced the standing outward current (ISO) at -20 mV by 106+/-17 pA, inhibiting a conductance (reversal, -95+/-4 mV) which displayed Goldman-Hodgkin-Katz outward rectification, supportive of a TASK-like K+ current. Since TASK channels are modulated by extracellular pH (pHo), we next investigated the pH sensitivity of ISO in Hepes-buffered ACSF. At pHo 7.3, DVN exhibited an ISO of 147+/-15 pA at -20 mV. Acidification to pHo 6.3 reduced ISO to 85+/-13 pA, whereas raising pHo to 8.5 increased ISO to 216+/-26 pA. At pHo 7.3, ISO was inhibited by BaCl2 (IC50 465 microM), but unaffected by ZnCl2 (100 microM). 5-HT (10 microM) reduced ISO by 114+/-17 pA at pHo 7.3, but at pHo 6.3 the 5-HT-induced inhibition of ISO was significantly smaller. The present data suggest that the excitatory effects of 5-HT on DVN are mediated in part by inhibition of a TASK-like, pH-sensitive K+ conductance. The pharmacological profile of this conductance excludes TASK-3 homomers, but rather implicates TASK-1-containing channels.
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