ArticleThe Journal of physiology2005
Water permeability of Na+-K+-2Cl- cotransporters in mammalian epithelial cells.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 74 citations in OpenAlex.
- Diuretic inhibition of experimental myopia implicates retinal ion-driven efflux in the regulation of ocular growth.Frontiers in medicine · 2026Article
- Osmolarity Controls Oscillatory Calcium Signaling to Reduce Autonomous Aldosterone Production in Zona Glomerulosa Cells.Endocrinology · 2025Article
- Navigating the multifaceted intricacies of the NaPhysiological reviews · 2024Review
- SWEET family transporters act as water conducting carrier proteins in plants.bioRxiv : the preprint server for biology · 2024Article
- Seasonal flexibility of the gut structure and physiology in Eremias multiocellata.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Non-Aquaporin Water Channels.Advances in experimental medicine and biology · 2023Article
- Cryo-EM structure of the human sodium-chloride cotransporter NCC.Science advances · 2022Article
- The choroid plexus and its role in the pathogenesis of neurological infections.Fluids and barriers of the CNS · 2022Review
- Neuronal Swelling: A Non-osmotic Consequence of Spreading Depolarization.Neurocritical care · 2021Article
- Cryo-EM structures ofAmerican journal of physiology. Cell physiology · 2020Article
- ECS Dynamism and Its Influence on Neuronal Excitability and Seizures.Neurochemical research · 2019Review
- Genetic and pharmacological inactivation of apical NaAmerican journal of physiology. Cell physiology · 2019Article
- Reciprocal Regulation of KCC2 Trafficking and Synaptic Activity.Frontiers in cellular neuroscience · 2019Review
- Directional Fluid Transport across Organ-Blood Barriers: Physiology and Cell Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Orexin-A promotes Glu uptake by OX1R/PKCα/ERK1/2/GLT-1 pathway in astrocytes and protects co-cultured astrocytes and neurons against apoptosis in anoxia/hypoglycemic injury in vitro.Molecular and cellular biochemistry · 2017Article
- Characterization of Na+-K+-2Cl- Cotransporter Activity in Rabbit Lacrimal Gland Duct Cells.Investigative ophthalmology & visual science · 2016Article
- Molecular pathophysiology of cerebral edema.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2016Review
- Blood cells and endothelial barrier function.Tissue barriers · 2015Article
- Review
- Novel determinants of the neuronal Cl(-) concentration.The Journal of physiology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Water transport properties of the Na+-K+-2Cl- cotransporter (NKCC) were studied in cultures of pigmented epithelial cells (PE) from the ciliary body of the eye. Here, the membrane that faces upwards contains NKCCs and can be subjected to rapid changes in bathing solution composition and osmolarity. The anatomy of the cultured cell layer was investigated by light and electron microscopy. The transport rate of the cotransporter was determined from the bumetanide-sensitive component of 86Rb+ uptake, and volume changes were derived from quenching of the fluorescent dye calcein. The water permeability (Lp) of the membrane was halved by the specific inhibitor bumetanide. The bumetanide-sensitive component of the water transport exhibited apparent saturation at osmotic gradients higher than 200 mosmol l-1. Cell shrinkages produced by NaCl or KCl were smaller than those elicited by equi-osmolar applications of mannitol, indicating reflection coefficients for these salts close to zero. The activation energy of the bumetanide-sensitive component of the Lp was 21 kcal mol-1, which is four times higher than that of an aqueous pore. The data suggest that osmotic transport via the cotransporter involves conformational changes of the cotransporter and interaction with Na+, K+ and Cl-. Similar measurements were performed on immortalized cell cultures from the thick ascending limb of the loop of Henle (TALH). Given similar overall transport rates of bumetanide-sensitive 86Rb+, the NKCCs of this tissue did not contribute any bumetanide-sensitive Lp. This suggests that the cotransporters of the two tissues are either different isoforms or the same cotransporter but in two different transport modes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.