Evidence map›Paper›PMID 16014736›Full record

ArticleToxicological sciences : an official journal of the Society of Toxicology2005

In vitro cytotoxicity of nanoparticles in mammalian germline stem cells.

Laura Braydich-Stolle, Saber Hussain, John J Schlager, Marie-Claude Hofmann

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicological sciences : an official journal of the Society of Toxicology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 226 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
226citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

226 citing papers in PubMed, 3 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. In vivo human time-exposure study of orally dosed commercial silver nanoparticles.Nanomedicine : nanotechnology, biology, and medicine · 2014
    Trial
  5. Trial
  6. Article
  7. Review
  8. Emerging nanomedicine for liver diseases treatment.Journal of nanobiotechnology · 2025
    Review
  9. Article
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  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
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  18. Article
  19. Article
  20. Review

166 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Laura Braydich-StolleDepartment of Biology, The University of Dayton, OH 45469, USA.
Saber Hussain
John J Schlager
Marie-Claude Hofmann

Funding

Isolation and Characterization of Testis Stem CellsR01HD044543 · NICHD · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI HOFMANN, MARIE-CLAUDE CATHERINE · 2005 to 2009
$1.4M
NICHD NIH HHS R01 HD044543
6 · The paper itself

Abstract

Gametogenesis is a complex biological process that is particularly sensitive to environmental insults such as chemicals. Many chemicals have a negative impact on the germline, either by directly affecting the germ cells, or indirectly through their action on the somatic nursing cells. Ultimately, these effects can inhibit fertility, and they may have negative consequences for the development of the offspring. Recently, nanomaterials such as nanotubes, nanowires, fullerene derivatives (buckyballs), and quantum dots have received enormous national attention in the creation of new types of analytical tools for biotechnology and the life sciences. Despite the wide application of nanomaterials, there is a serious lack of information concerning their impact on human health and the environment. Thus, there are limited studies available on toxicity of nanoparticles for risk assessment of nanomaterials. The purpose of this study was to assess the suitability of a mouse spermatogonial stem cell line as a model to assess nanotoxicity in the male germline in vitro. The effects of different types of nanoparticles on these cells were evaluated by light microscopy, and by cell proliferation and standard cytotoxicity assays. Our results demonstrate a concentration-dependent toxicity for all types of particles tested, whereas the corresponding soluble salts had no significant effect. Silver nanoparticles were the most toxic while molybdenum trioxide (MoO(3)) nanoparticles were the least toxic. Our results suggest that this cell line provides a valuable model with which to assess the cytotoxicity of nanoparticles in the germ line in vitro.

Indexed as

NanotechnologyAnimalsApoptosisCadmium CompoundsCarbonatesCell LineCell ProliferationCell SurvivalDose-Response Relationship, DrugDrug CombinationsL-Lactate DehydrogenaseMaleMiceMitochondriaMolybdenumNanostructures3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium5-ethylphenazineCadmium Compoundscadmium oxideCarbonatesDrug CombinationsL-Lactate DehydrogenaseMolybdenummolybdenum trioxideOxidesPhenazinessilver carbonateSilver CompoundsTetrazolium SaltsThiazoles

Identifiers

PMID16014736
PMCPMC2911231

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.