ArticleCurrent genetics2005
Identification and characterization of upstream open reading frames (uORF) in the 5' untranslated regions (UTR) of genes in Saccharomyces cerevisiae.
Article in Current genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 76 citations in OpenAlex.
- Interaction of the La-related protein Slf1 with colliding ribosomes maintains translation of oxidative-stress responsive mRNAs.Nucleic acids research · 2023Article
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- A standard knockout procedure alters expression of adjacent loci at the translational level.Nucleic acids research · 2021Article
- The role of upstream open reading frames in translation regulation in the apicomplexan parasitesParasitology · 2021Review
- Impact of uORFs in mediating regulation of translation in stress conditions.BMC molecular and cell biology · 2021Article
- Suppression of Ribosomal Pausing by eIF5A Is Necessary to Maintain the Fidelity of Start Codon Selection.Cell reports · 2019Article
- Impacts of uORF codon identity and position on translation regulation.Nucleic acids research · 2019Article
- Translational regulation in response to stress in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2019Review
- Conserved non-AUG uORFs revealed by a novel regression analysis of ribosome profiling data.Genome research · 2018Article
- Zap1-dependent transcription from an alternative upstream promoter controls translation of RTC4 mRNA in zinc-deficient Saccharomyces cerevisiae.Molecular microbiology · 2017Article
- Classification and function of small open reading frames.Nature reviews. Molecular cell biology · 2017Article
- Comprehensive identification of translation start sites by tetracycline-inhibited ribosome profiling.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Review
- A novel strategy to produce sweeter tomato fruits with high sugar contents by fruit-specific expression of a single bZIP transcription factor gene.Plant biotechnology journal · 2016Article
- Article
- Emerging evidence for functional peptides encoded by short open reading frames.Nature reviews. Genetics · 2014Review
- Deciphering the rules by which 5'-UTR sequences affect protein expression in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing.Genome research · 2013Article
- Extensive transcript diversity and novel upstream open reading frame regulation in yeast.G3 (Bethesda, Md.) · 2013Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have taken advantage of recently sequenced hemiascomycete fungal genomes to computationally identify additional genes potentially regulated by upstream open reading frames (uORFs). Our approach is based on the observation that the structure, including the uORFs, of the post-transcriptionally uORF regulated Saccharomyces cerevisiae genes GCN4 and CPA1 is conserved in related species. Thirty-eight candidate genes for which uORFs were found in multiple species were identified and tested. We determined by 5' RACE that 15 of these 38 genes are transcribed. Most of these 15 genes have only a single uORF in their 5' UTR, and the length of these uORFs range from 3 to 24 codons. We cloned seven full-length UTR sequences into a luciferase (LUC) reporter system. Luciferase activity and mRNA level were compared between the wild-type UTR construct and a construct where the uORF start codon was mutated. The translational efficiency index (TEI) of each construct was calculated to test the possible regulatory function on translational level. We hypothesize that uORFs in the UTR of RPC11, TPK1, FOL1, WSC3, and MKK1 may have translational regulatory roles while uORFs in the 5' UTR of ECM7 and IMD4 have little effect on translation under the conditions tested.
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