ArticleImmunology2005
Developing dendritic cells become 'lacy' cells packed with fat and glycogen.
Article in Immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Dendritic cells: understanding ontogeny, subsets, functions, and their clinical applications.Molecular biomedicine · 2025Review
- Dendritic cell maturation in cancer.Nature reviews. Cancer · 2025Review
- Precise targeting of lipid metabolism in the era of immuno-oncology and the latest advances in nano-based drug delivery systems for cancer therapy.Acta pharmaceutica Sinica. B · 2024Review
- Fatty acid oxidation in immune function.Frontiers in immunology · 2024Review
- Lipid metabolism in dendritic cell biology.Immunological reviews · 2023Review
- Abnormal lipid metabolism in epidermal Langerhans cells mediates psoriasis-like dermatitis.JCI insight · 2022Article
- Metabolomic and lipidomic signatures associated with activation of human cDC1 (BDCA3Immunology · 2022Article
- Fa(c)t checking: How fatty acids shape metabolism and function of macrophages and dendritic cells.European journal of immunology · 2021Review
- Lipid Metabolism and Cancer Immunotherapy: Immunosuppressive Myeloid Cells at the Crossroad.International journal of molecular sciences · 2020Review
- Article
- Lung adenocarcinoma-intrinsic GBE1 signaling inhibits anti-tumor immunity.Molecular cancer · 2019Article
- Immunometabolism: A new target for improving cancer immunotherapy.Advances in cancer research · 2019Review
- Metabolic Control of Dendritic Cell Functions: Digesting Information.Frontiers in immunology · 2019Review
- Dendritic Cells Require PINK1-Mediated Phosphorylation of BCKDE1α to Promote Fatty Acid Oxidation for Immune Function.Frontiers in immunology · 2019Article
- Lipid Accumulation in Peripheral Blood Dendritic Cells and Anticancer Immunity in Patients with Lung Cancer.Journal of immunology research · 2018Article
- Emerging Roles of Cellular Metabolism in Regulating Dendritic Cell Subsets and Function.Frontiers in cell and developmental biology · 2018Review
- Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.Cell metabolism · 2017Article
- The bioenergetics of inflammation: insights into obesity and type 2 diabetes.European journal of clinical nutrition · 2017Review
- Molecular mechanisms involved in dendritic cell dysfunction in cancer.Cellular and molecular life sciences : CMLS · 2017Review
- Tumor-derived factors modulating dendritic cell function.Cancer immunology, immunotherapy : CII · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
On maturation, dendritic cells (DCs) become highly active cells equipped for antigen uptake, migration and clustering and activation of T cells. We therefore asked whether DCs acquire fat and glycogen stores as they mature. DCs were generated from mouse bone marrow stem cells by culturing with granulocyte-macrophage colony-stimulating factor (GM-CSF) for 7-8 days. Stimulation of the DCs with lipopolysaccharide (LPS) for the last 24 hr of culture, or exposure to 1-15 ng/ml of interleukin (IL)-4 during development, resulted in production of DCs not only with an increased ability to stimulate T cells but also with an increasingly lacy appearance on transmission electron microscopy, with multiple unstained areas in the cytoplasm. This changed morphology was associated with the presence of increasing amounts of fat and glycogen, identified by Sudan Black and periodic acid leukofushin/Schiff (PAS) staining, respectively. Lacy DCs up-regulated type 1 and type 2 scavenger receptors, providing possible mechanisms contributing to these changes. Lacy DCs were found occasionally amongst freshly isolated splenic and lymph node DCs. DCs can be isolated from human adipose tissue, and we tested whether lacy DCs acquiring fat and glycogen were present in mouse omentum. CD45+ cells migrating from the omentum expressed specific DC markers CD11c and 33D1, costimulatory molecules and major histocompatibility complex (MHC) class II, and most showed darkly staining fat inclusions. Thus, during development, DCs can acquire large amounts of fat and glycogen, accumulation of which is promoted by antigen exposure and modulated by the cytokine milieu and location, and which may act as a link between energy stores and immune function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.