Evidence map›Paper›PMID 16007532›Full record

ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2005

Improved diagnosis of the etiology of community-acquired pneumonia with real-time polymerase chain reaction.

Kate E Templeton, Sitha A Scheltinga, Willian C J F M van den Eeden, A Willy Graffelman, Peterhans J van den Broek, Eric C J Claas

Abstract read
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In one paragraph

Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers, 8 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
153citing papers in PubMed, 8 pooled it
10.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

153 citing papers in PubMed, 8 syntheses or guidelines pooled it, 337 citations in OpenAlex.

  1. Pooled it
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  3. Systematic review of respiratory viral pathogens identified in adults with community-acquired pneumonia in Europe.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017
    Pooled it
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  5. Viral infection in community-acquired pneumonia: a systematic review and meta-analysis.European respiratory review : an official journal of the European Respiratory Society · 2016
    Pooled it
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  7. The role of Streptococcus pneumoniae in community-acquired pneumonia among adults in Europe: a meta-analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013
    Pooled it
  8. Infectious Diseases Society of America/American Thoracic Society consensus guidelines on the management of community-acquired pneumonia in adults.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2007
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93 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Kate E TempletonDepartment of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.
Sitha A Scheltinga
Willian C J F M van den Eeden
A Willy Graffelman
Peterhans J van den Broek
Eric C J Claas
Leiden University Medical Center · NLCzech Academy of Sciences, Institute of Microbiology · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCommunity-acquired pneumonia (CAP) remains an important cause of morbidity and mortality. Polymerase chain reaction (PCR) has been shown to be more sensitive than current standard microbiological methods and, therefore, may improve the accuracy of microbiological diagnosis for patients with CAP.

methodsConventional detection techniques and multiplex real-time PCR for atypical bacteria and respiratory viruses were performed on samples collected from 105 adults enrolled in a prospective study. An infiltrate was visible on each patient's chest radiograph, and a pneumonia severity index score was determined for each patient.

resultsMicrobiological diagnoses were determined for 52 (49.5%) of 105 patients by conventional techniques and for 80 (76%) of 105 patients by real-time PCR. The time to obtain the result of real-time PCR could be reduced to 6 h. PCR methodology was significantly more sensitive for the detection of atypical pathogens and viruses (P< or =.001). Respiratory viral infections and mixed infections were detected in 15 (14.2%) and 3 (2.8%) of 105 patients, respectively, by conventional methods, but were detected in 59 (56.2%) and 28 (26.5%) of 105, respectively, by real-time PCR. Presence of a mixed infection was significantly associated with severe pneumonia (P=.002). Human rhinoviruses and coronaviruses, OC43 and 229E, were frequently identified pathogens.

conclusionsThe combined real-time PCR assay is more sensitive for diagnosis of the main viruses and atypical bacteria that cause CAP compared with conventional methods, and obtains results in a clinically relevant time period.

Indexed as

AdolescentAdultAgingCommunity-Acquired InfectionsFemaleHumansMaleMiddle AgedNetherlandsPneumoniaPolymerase Chain ReactionRisk Factors

Identifiers

PMID16007532
PMCPMC7107904
OpenAlexW2151082975

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.