ArticleJournal of molecular evolution2005
Recombination during in vitro evolution.
Article in Journal of molecular evolution, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Design and Experimental Evolution of trans-Splicing Group I Intron Ribozymes.Molecules (Basel, Switzerland) · 2017Review
- Sex in a test tube: testing the benefits of in vitro recombination.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- From formamide to RNA, the path is tenuous but continuous.Life (Basel, Switzerland) · 2015Article
- RNA synthesis by in vitro selected ribozymes for recreating an RNA world.Life (Basel, Switzerland) · 2015Review
- Primal eukaryogenesis: on the communal nature of precellular States, ancestral to modern life.Life (Basel, Switzerland) · 2012Article
- Exploiting models of molecular evolution to efficiently direct protein engineering.Journal of molecular evolution · 2011Article
- Article
- Unbiased in vitro selection reveals the unique character of the self-cleaving antigenomic HDV RNA sequence.Nucleic acids research · 2006Article
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2 authors.
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Abstract
Recombination, the swapping of large portions of genetic information between and among parental genotypes, can be applied to in vitro evolution experiments on functional nucleic acids. Both homologous and heterologous recombination can be achieved using standard laboratory techniques. In many cases, recombination can allow for the discovery of a ribozyme or DNAzyme phenotype that would not likely be encountered by reliance on point mutations alone. In addition, recombination can often aid in the discovery of global optima in sequence space and/or lessen the number of generations it would take to reach optima. Recombination is most efficiently used in combination with point mutations and applied after the first couple of rounds of selection but before high-fitness genotypes dominate the selection. The "recombination zone" describes that region of sequence space-defined by the residues that will ultimately participate in the function of the winning nucleic acid(s)-where recombination is expected to be the most beneficial in the search for high-fitness genotypes.
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