ArticleMarine biotechnology (New York, N.Y.)
Species-specific detection and quantification of toxic marine dinoflagellates Alexandrium tamarense and A. catenella by Real-time PCR assay.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- An On-Farm Workflow for Predictive Management of Paralytic Shellfish Toxin-Producing Harmful Algal Blooms for the Aquaculture Industry.Environmental science & technology · 2024Article
- Development of a Method for DetectingToxins · 2022Article
- Application of loop-mediated isothermal amplification combined with lateral flow dipstick to rapid and sensitive detection of Alexandrium catenella.Environmental science and pollution research international · 2020Article
- Evaluation of sxtA and rDNA qPCR assays through monitoring of an inshore bloom of Alexandrium catenella Group 1.Scientific reports · 2019Article
- The Genetic Basis of Toxin Biosynthesis in Dinoflagellates.Microorganisms · 2019Review
- qPCR Assays for the Detection and Quantification of Multiple Paralytic Shellfish Toxin-Producing Species ofFrontiers in microbiology · 2018Article
- Molecular Techniques for the Detection of Organisms in Aquatic Environments, with Emphasis on Harmful Algal Bloom Species.Sensors (Basel, Switzerland) · 2017Review
- qPCR assays forPhycologia · 2017Article
- High Specificity of a Quantitative PCR Assay Targeting a Saxitoxin Gene for Monitoring Toxic Algae Associated with Paralytic Shellfish Toxins in the Yellow Sea.Applied and environmental microbiology · 2015Article
- Molecular detection of a potentially toxic diatom species.International journal of environmental research and public health · 2015Article
- Biology of the Marine Heterotrophic DinoflagellateMicroorganisms · 2013Review
- The quantitative real-time PCR applications in the monitoring of marine harmful algal bloom (HAB) species.Environmental science and pollution research international · 2013Article
- Article
- Molecular detection and species identification of Alexandrium (Dinophyceae) causing harmful algal blooms along the Chilean coastline.AoB PLANTS · 2012Article
- Examination of the seasonal dynamics of the toxic dinoflagellate Alexandrium catenella at Redondo Beach, California, by quantitative PCR.Applied and environmental microbiology · 2011Article
- sxtA-based quantitative molecular assay to identify saxitoxin-producing harmful algal blooms in marine waters.Applied and environmental microbiology · 2011Article
- Real-time PCR detection of Dinophysis species in Irish coastal waters.Marine biotechnology (New York, N.Y.) · 2010Article
- A quantitative real-time PCR assay for the identification and enumeration of Alexandrium cysts in marine sediments.Deep-sea research. Part II, Topical studies in oceanography · 2010Article
- Multiple simultaneous detection of Harmful Algal Blooms (HABs) through a high throughput bead array technology, with potential use in phytoplankton community analysis.Harmful algae · 2009Article
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Authors and funding
2 authors.
Funding
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Abstract
A Real-time polymerase chain reaction (PCR) assay was designed and evaluated for rapid detection and quantification of the toxic dinoflagellates Alexandrium catenella and A. tamarense, which cause paralytic shellfish poisoning. Two sets of PCR primers and fluorogenic probes targeting these two species were derived from the sequence of 28S ribosomal DNA. PCR specificity was examined in closely related Alexandrium spp. and many other microalgae. A. catenella-specific primers and probe detected the PCR amplification only from A. catenella strains, and nonspecific signals were not detected from any microalgae. Also, A. tamarense-specific primers and probe also detected the targeted species, suggesting the strict species specificity of each PCR. This assay could detect one cell of each species, showing its high sensitivity. Moreover, using the developed standard curves, A. tamarense and A. catenella could be quantified in agreement with the quantification by optical microscopy. The performance characteristics of species specificity, sensitivity, and rapidity suggest that this method is applicable to the monitoring of the toxic A. tamarense and A. catenella.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.