ArticleThe Journal of clinical investigation2005
Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor.
Article in The Journal of clinical investigation, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 95 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Uncovering the 'ORIGINS' of Diabetes
Investigating the Effects of Aerobic and Resistance Training in Vivo on Skeletal Muscle Metabolism in Vitro in Primary Human Muscle Cells (MoTrMyo)
Who cites it
95 citing papers in PubMed, 1 synthesis or guideline pooled it, 212 citations in OpenAlex.
- Association Between Adipose Tissue Characteristics and Metabolic Flexibility in Humans: A Systematic Review.Frontiers in nutrition · 2021Pooled it
- Sustained fasting glucose oxidation and postprandial lipid oxidation associated with reduced insulin dose in type 2 diabetes with sodium-glucose cotransporter 2 inhibitor: A randomized, open-label, prospective study.Journal of diabetes investigation · 2019Trial
- Perilipin 3 Differentially Regulates Skeletal Muscle Lipid Oxidation in Active, Sedentary, and Type 2 Diabetic Males.The Journal of clinical endocrinology and metabolism · 2015Trial
- Nine months of combined training improves ex vivo skeletal muscle metabolism in individuals with type 2 diabetes.The Journal of clinical endocrinology and metabolism · 2013Trial
- Sex steroids affect triglyceride handling, glucose-dependent insulinotropic polypeptide, and insulin sensitivity: a 1-week randomized clinical trial in healthy young men.Diabetes care · 2010Trial
- Skeletal muscle NAMPT is induced by exercise in humans.American journal of physiology. Endocrinology and metabolism · 2010Trial
- Dietary sugars stimulate fatty acid synthesis in adults.The Journal of nutrition · 2008Trial
- Calorie restriction increases muscle mitochondrial biogenesis in healthy humans.PLoS medicine · 2007Trial
- miRNAs as cell-intrinsic epigenetic regulators in muscle in type 2 diabetes.Signal transduction and targeted therapy · 2026Article
- Downregulation of microRNA-494 drives mitochondrial biogenesis and function in trained muscle.Experimental physiology · 2026Article
- mTORC1 signalling and protein synthesis are elevated in response to amino acids in human myotubes obtained from young, old, and old trained men.In vitro cellular & developmental biology. Animal · 2026Article
- Insulin Resistance in Type 1 Diabetes: Pathophysiological, Clinical, and Therapeutic Relevance.Endocrine reviews · 2025Review
- Vitamin D and its receptor in skeletal muscle are associated with muscle disease manifestation, lipid metabolism and physical fitness of patients with myositis.Arthritis research & therapy · 2025Article
- New Frontiers: Umbilical Cord Mesenchymal Stem Cells Uncover Developmental Roots and Biological Underpinnings of Obesity Susceptibility.Current obesity reports · 2025Review
- Aging delays the suppression of lipolysis and fatty acid oxidation in the postprandial period.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Fat burning capacity in a mixed macronutrient meal protocol does not reflect metabolic flexibility in women who are overweight or obese.medRxiv : the preprint server for health sciences · 2024Article
- Sedentary time associates detrimentally and physical activity beneficially with metabolic flexibility in adults with metabolic syndrome.American journal of physiology. Endocrinology and metabolism · 2024Article
- From Obesity-Induced Low-Grade Inflammation to Lipotoxicity and Mitochondrial Dysfunction: Altered Multi-Crosstalk between Adipose Tissue and Metabolically Active Organs.Antioxidants (Basel, Switzerland) · 2023Review
- The Role of Mitochondrial Adaptation and Metabolic Flexibility in the Pathophysiology of Obesity and Insulin Resistance: an Updated Overview.Current obesity reports · 2021Review
- Maternal metabolic health drives mesenchymal stem cell metabolism and infant fat mass at birth.JCI insight · 2021Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic flexibility of skeletal muscle, that is, the preference for fat oxidation (FOx) during fasting and for carbohydrate oxidation in response to insulin, is decreased during insulin resistance. The aim of this study was to test the hypothesis that the capacity of myotubes to oxidize fat in vitro reflects the donor's metabolic characteristics. Insulin sensitivity (IS) and metabolic flexibility of 16 healthy, young male subjects was determined by euglycemic hyperinsulinemic clamp. Muscle samples were obtained from vastus lateralis, cultured, and differentiated into myotubes. In human myotubes in vitro, we measured suppressibility (glucose suppression of FOx) and adaptability (an increase in FOx in the presence of high palmitate concentration). We termed these dynamic changes in FOx metabolic switching. In vivo, metabolic flexibility was positively correlated with IS and maximal oxygen uptake and inversely correlated with percent body fat. In vitro suppressibility was inversely correlated with IS and metabolic flexibility and positively correlated with body fat and fasting FFA levels. Adaptability was negatively associated with percent body fat and fasting insulin and positively correlated with IS and metabolic flexibility. The interindividual variability in metabolic phenotypes was preserved in human myotubes separated from their neuroendocrine environment, which supports the hypothesis that metabolic switching is an intrinsic property of skeletal muscle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.