ArticleOncogene2005
Combined affinity labelling and mass spectrometry analysis of differential cell surface protein expression in normal and prostate cancer cells.
Article in Oncogene, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Neuronal Protection by Ha-RAS-GTPase Signaling through Selective Downregulation of Plasmalemmal Voltage-Dependent Anion Channel-1.International journal of molecular sciences · 2024Article
- Invadopodia play a role in prostate cancer progression.BMC cancer · 2022Article
- Paired Expression Analysis of Tumor Cell Surface Antigens.Frontiers in oncology · 2017Article
- Ecto-5'-nucleotidase expression is associated with the progression of renal cell carcinoma.Oncology letters · 2015Article
- Plasma membrane proteomics identifies biomarkers associated with MMSET overexpression in T(4;14) multiple myeloma.Oncotarget · 2013Article
- A2B adenosine receptor blockade inhibits growth of prostate cancer cells.Purinergic signalling · 2013Article
- Identification of markers of prostate cancer progression using candidate gene expression.British journal of cancer · 2012Article
- Ectonucleotidases in tumor cells and tumor-associated immune cells: an overview.Journal of biomedicine & biotechnology · 2012Review
- Conditions of tumor-associated antigens as a proper target for therapeutic antibodies against solid cancers.Journal of stem cells & regenerative medicine · 2011Article
- RNAi-mediated CD73 suppression induces apoptosis and cell-cycle arrest in human breast cancer cells.Cancer science · 2010Article
- Anti-CD73 antibody therapy inhibits breast tumor growth and metastasis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Identification of calcium-binding proteins associated with the human sperm plasma membrane.Reproductive biology and endocrinology : RB&E · 2010Article
- Heat shock proteins on the human sperm surface.Journal of reproductive immunology · 2010Article
- Cell surface proteomics identifies molecules functionally linked to tumor cell intravasation.The Journal of biological chemistry · 2008Article
- Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.The Journal of biological chemistry · 2008Article
- Quantitative analysis of surface plasma membrane proteins of primary and metastatic melanoma cells.Journal of proteome research · 2008Article
- VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation.Molecular and cellular biochemistry · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Differences in the expression of cell surface proteins between a normal prostate epithelial (1542-NP2TX) and a prostate cancer cell line (1542-CP3TX) derived from the same patient were investigated. A combination of affinity chromatographic purification of biotin-tagged surface proteins with mass spectrometry analysis identified 26 integral membrane proteins and 14 peripheral surface proteins. The findings confirm earlier reports of altered expression in prostate cancer for several cell surface proteins, including ALCAM/CD166, the Ephrin type A receptor, EGFR and the prostaglandin F2 receptor regulatory protein. In addition, several novel findings of differential expression were made, including the voltage-dependent anion selective channel proteins Porin 1 and 2, ecto-5'-nucleotidase (CD73) and Scavenger receptor B1. Cell surface protein expression changed both qualitatively and quantitatively when the cells were grown in the presence of either or both interferon INFalpha and INFgamma. Costimulation with type I and II interferons had additive or synergistic effects on the membrane density of several, mainly peripherally attached surface proteins. Concerted upregulation of surface exposed antigens may be of benefit in immuno-adjuvant-based treatment of interferon-responsive prostate cancer. In conclusion, this study demonstrates that differences in the expression of membrane proteins between normal and prostate cancer cells are reproducibly detectable following vectorial labelling with biotin, and that detailed analysis of extracellular-induced surface changes can be achieved by combining surface-specific labelling with high-resolution two-dimensional gel electrophoresis and mass spectrometry.
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Registered trials
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