ArticleProceedings of the National Academy of Sciences of the United States of America2005
Genomic imprinting recapitulated in the human beta-globin locus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Germline epigenome editing identifies H3K9me3 as a mediator of intergenerational DNA methylation recovery in mice.Nature communications · 2025Article
- Imprinted DNA methylation of the H19 ICR is established and maintained in vivo in the absence of Kaiso.Epigenetics & chromatin · 2024Article
- Five nucleotides found in RCTG motifs are essential for post-fertilization methylation imprinting of the H19 ICR in YAC transgenic mice.Nucleic acids research · 2023Article
- The transgenic IG-DMR sequence of the mouse Dlk1-Dio3 domain acquired imprinted DNA methylation during the post-fertilization period.Epigenetics & chromatin · 2023Article
- DNA sequence and chromatin modifiers cooperate to confer epigenetic bistability at imprinting control regions.Nature genetics · 2022Article
- Orientation of mouse H19 ICR affects imprinted H19 gene expression through promoter methylation-dependent and -independent mechanisms.Communications biology · 2021Article
- Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus.Epigenetics & chromatin · 2020Article
- Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice.Epigenetics & chromatin · 2018Article
- Fate of methylated/unmethylated H19 imprinting control region after paternal and maternal pronuclear injection.Experimental animals · 2017Article
- Imprinted DNA methylation reconstituted at a non-imprinted locus.Epigenetics & chromatin · 2016Article
- Insertion of an imprinted insulator into the IgH locus reveals developmentally regulated, transcription-dependent control of V(D)J recombination.Molecular and cellular biology · 2015Article
- The H19 imprinting control region mediates preimplantation imprinted methylation of nearby sequences in yeast artificial chromosome transgenic mice.Molecular and cellular biology · 2013Article
- New insights into establishment and maintenance of DNA methylation imprints in mammals.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Article
- Promoter cross-talk via a shared enhancer explains paternally biased expression of Nctc1 at the Igf2/H19/Nctc1 imprinted locus.Nucleic acids research · 2013Article
- More than insulator: multiple roles of CTCF at the H19-Igf2 imprinted domain.Frontiers in genetics · 2012Article
- Epigenetic transgenerational actions of vinclozolin on promoter regions of the sperm epigenome.PloS one · 2010Article
- Article
- H19 imprinting control region methylation requires an imprinted environment only in the male germ line.Molecular and cellular biology · 2010Article
- A randomly integrated transgenic H19 imprinting control region acquires methylation imprinting independently of its establishment in germ cells.Molecular and cellular biology · 2009Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
A subset of genes in mammals are subject to genomic imprinting. The mouse H19 gene, for example, is active only when maternally inherited and the neighboring Igf2 gene is paternally expressed. This imprinted expression pattern is regulated by the imprinting control region (ICR) upstream of the H19 gene. A maternally inherited H19 ICR inhibits Igf2 gene activation by the downstream enhancer due to its insulator function while it suppresses H19 gene transcription by promoter DNA methylation when paternally inherited. These parent-of-origin specific functions depend on the allele-specific methylation of the ICR DNA, which is established during gametogenesis. Therefore, the ICR may also function as a landmark for epigenetic modifications. To examine whether the ICR confers these activities autonomously, we introduced a 2.9-kbp ICR-containing DNA fragment into a human beta-globin yeast artificial chromosome at the 3' end of the locus control region and established transgenic mouse lines. Expression of all of the beta-like globin genes was higher when the transgene was paternally inherited. In accord with this result, transgenic ICR DNA from nucleated erythrocytes was more heavily methylated when paternally transmitted. Chromatin immunoprecipitation assays confirmed that CCCTC binding factor is preferentially recruited to the maternal transgenic ICR in vivo. Surprisingly however, the parent-of-origin specific methylation pattern was not observed in germ cell DNA in testis, demonstrating that methylation was established after fertilization. Thus, the ICR autonomously recapitulated imprinting within the normally nonimprinted transgenic beta-globin gene locus, but the temporal establishment of imprinting methylation differs from that at the endogenous Igf2/H19 locus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.