ArticleEukaryotic cell2005
Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 127 citations in OpenAlex.
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- Regulation of TRI5 expression and deoxynivalenol biosynthesis by a long non-coding RNA in Fusarium graminearum.Nature communications · 2024Article
- UncoveringmBio · 2022Article
- Development of versatile and efficient genetic tools for the marine-derived fungus Aspergillus terreus RA2905.Current genetics · 2022Article
- Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues.Microbial cell factories · 2022Article
- Review
- Control of Development, Secondary Metabolism and Light-Dependent Carotenoid Biosynthesis by the Velvet Complex ofGenetics · 2019Article
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- New Plasmids forFrontiers in microbiology · 2018Article
- Quantitative Analysis of Proteome Modulations in Alveolar Epithelial Type II Cells in Response to PulmonaryMolecular & cellular proteomics : MCP · 2017Article
- HisB as novel selection marker for gene targeting approaches in Aspergillus niger.BMC microbiology · 2017Article
- Expression and chromatin structures of cellulolytic enzyme gene regulated by heterochromatin protein 1.Biotechnology for biofuels · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detailed evaluation of gene functions in an asexual fungus requires advanced methods of molecular biology. For the generation of targeted gene deletions in the opportunistic pathogen Aspergillus fumigatus we designed a novel blaster module allowing dominant selection of transformants due to resistance to phleomycin as well as dominant (counter)selection of a Cre recombinase-mediated marker excision event. For validation purposes we have deleted the A. fumigatus pabaA gene in a wild-type isolate by making use of this cassette. The resulting pabaA::loxP strain served as the recipient for subsequent targeting of the velvet locus. Homologous reconstitution of the deleted gene was performed by an allele whose expression is driven in a nitrogen source-dependent manner, as validated by Northern analyses. Overexpression of the veA locus in A. fumigatus does not result in any obvious phenotype, whereas the sporulation capacities of the veA null mutant are reduced on nitrate-containing medium, a phenotype that is completely restored in the reconstituted strain.
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