ArticleThe Journal of physiology2005
Enhancement of spontaneous synaptic activity in rat Purkinje neurones by ATP during development.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Cerebellar deep brain stimulation rescues Purkinje cell mitochondrial density in a genetic mouse model of cerebellar ataxia.Brain research bulletin · 2026Article
- Specific Temporal Distribution and Subcellular Localization of a Functional Vesicular Nucleotide Transporter (VNUT) in Cerebellar Granule Neurons.Frontiers in pharmacology · 2017Article
- Article
- Purinergic signalling during development and ageing.Purinergic signalling · 2015Review
- Depolarization-induced depression of inhibitory transmission in cerebellar Purkinje cells.Physiological reports · 2013Article
- Purinergic modulation of granule cells.Cerebellum (London, England) · 2012Review
- Intrinsic properties and mechanisms of spontaneous firing in mouse cerebellar unipolar brush cells.The Journal of physiology · 2007Article
- Glutamatergic and purinergic receptor-mediated calcium transients in Bergmann glial cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- P2 receptors and neuronal injury.Pflugers Archiv : European journal of physiology · 2006Review
- Nucleotide signaling in nervous system development.Pflugers Archiv : European journal of physiology · 2006Review
- Modulation of synaptic activity in Purkinje neurons by ATP.Cerebellum (London, England) · 2006Review
- Interactions between Purkinje neurones and Bergmann glia.Cerebellum (London, England) · 2006Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The establishment of functional synaptic connections and activity is a pivotal process in the development of neuronal networks. We have studied the synaptic activity in the developing rat cerebellum, and the contribution mediated by purinergic receptors. The mean frequency of the spontaneous postsynaptic currents (sPSCs) recorded with the whole-cell patch-clamp technique from Purkinje neurones in acute brain slices at room temperature, increased fourfold from 4.4+/-0.8 Hz at postnatal day 9/10 (n=23) to 17.8+/-1.6 Hz at postnatal day 17-20 (p17-p20; n=113; P<0.01). ATP, which increased the frequency of sPSCs by up to 100% (EC50=18 microM) in the third postnatal week, started to modulate the synaptic activity during the second postnatal week, which was determined by three processes: (1) the appearance of functional ATP receptors during p10-p12, (2) the enhancement of the sPSC frequency by endogenous ATP release becoming apparent after inhibition of ecto-ATPases by 6-N,N-diethyl-beta,gamma-dibromomethylene-D-adenosine-5-triphosphate (ARL67156; 50 microM) at p11-p12, and (3) with tonic stimulation of purinoceptors at p14, as revealed by the P2 receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS, 10 microM). ATP had a similar effect at later stages (p24-p27) and at 35 degrees C. Our results suggest that endogenous release of ATP starts to enhance the synaptic activity in Purkinje neurones by the end of the second postnatal week.
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