ArticlePlanta2005
Functional characterization of Gossypium hirsutum profilin 1 gene (GhPFN1) in tobacco suspension cells. Characterization of in vivo functions of a cotton profilin gene.
Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- The 4Fs of cotton: genome editing of cotton for fiber, food, feed, and fuel to achieve zero hunger.Frontiers in genome editing · 2024Review
- Cotton fiber as a model for understanding shifts in cell development under domestication.Frontiers in plant science · 2023Review
- Transcriptional loss of domestication-driven cytoskeletal GhPRF1 gene causes defective floral and fiber development in cotton (Gossypium).Plant molecular biology · 2021Article
- G65V Substitution in Actin Disturbs Polymerization Leading to Inhibited Cell Elongation in Cotton.Frontiers in plant science · 2019Article
- Article
- OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice.Scientific reports · 2017Article
- Domestication-driven Gossypium profilin 1 (GhPRF1) gene transduces early flowering phenotype in tobacco by spatial alteration of apical/floral-meristem related gene expression.BMC plant biology · 2016Article
- Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers.Proteomes · 2016Article
- Profilin-Dependent Nucleation and Assembly of Actin Filaments Controls Cell Elongation in Arabidopsis.Plant physiology · 2016Article
- Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages.BMC genomics · 2012Article
- Characterization of PROFILIN genes from allotetraploid (Gossypium hirsutum) cotton and its diploid progenitors and expression analysis in cotton genotypes differing in fiber characteristics.Molecular biology reports · 2012Article
- Characterization of profilin polymorphism in pollen with a focus on multifunctionality.PloS one · 2012Article
- Parallel up-regulation of the profilin gene family following independent domestication of diploid and allopolyploid cotton (Gossypium).Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- BENT UPPERMOST INTERNODE1 encodes the class II formin FH5 crucial for actin organization and rice development.The Plant cell · 2011Article
- RICE MORPHOLOGY DETERMINANT encodes the type II formin FH5 and regulates rice morphogenesis.The Plant cell · 2011Article
- Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.PloS one · 2011Article
- Gene expression in developing fibres of Upland cotton (Gossypium hirsutum L.) was massively altered by domestication.BMC biology · 2010Article
- The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton.Plant molecular biology · 2010Article
- Arabidopsis formin3 directs the formation of actin cables and polarized growth in pollen tubes.The Plant cell · 2009Article
- Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cotton fiber is an extremely long plant cell. Fiber elongation is a complex process and the genes that are crucial for elongation are largely unknown. We previously cloned a cDNA encoding an isoform of cotton profilin and found that the gene (designated GhPFN1) was preferentially expressed in cotton fibers. In the present study, we have further analyzed the expression pattern of GhPFN1 during fiber development and studied its cellular function using tobacco suspension cells as an experimental system. We report that expression of GhPFN1 is tightly associated with fast elongation of cotton fibers whose growth requires an intact actin cytoskeleton. Overexpression of GhPFN1 in the transgenic tobacco cells was correlated with the formation of elongated cells that contained thicker and longer microfilament cables. Quantitative analyses revealed a 2.5-3.6 fold increase in total profilin levels and a 1.6-2.6 fold increase in the F-actin levels in six independent transgenic lines. In addition to the effect on cell elongation, we also observed delayed cell cycle progression and a slightly lower mitotic index in the transgenic cells. Based on these data, we propose that GhPFN1 may play a critical role in the rapid elongation of cotton fibers by promoting actin polymerization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.