ArticleApplied and environmental microbiology2005
Multiple-mutation reaction: a method for simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Reviewing advancement inFrontiers in microbiology · 2024Review
- Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.The EMBO journal · 2023Article
- Functional Redundancy and Specialization of the Conserved Cold Shock Proteins inMicroorganisms · 2021Article
- Characterization of an Immunoglobulin Binding Protein (IbpM) FromFrontiers in microbiology · 2020Article
- The heme-sensitive regulator SbnI has a bifunctional role in staphyloferrin B production byThe Journal of biological chemistry · 2019Article
- Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis.Nucleic acids research · 2018Article
- Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh inThe Journal of biological chemistry · 2018Article
- SbnI is a free serine kinase that generatesThe Journal of biological chemistry · 2018Article
- Identification of the Components Involved in Cyclic Di-AMP Signaling inFrontiers in microbiology · 2017Article
- Deciphering the Substrate Specificity of SbnA, the Enzyme Catalyzing the First Step in Staphyloferrin B Biosynthesis.Biochemistry · 2016Article
- Network of Surface-Displayed Glycolytic Enzymes in Mycoplasma pneumoniae and Their Interactions with Human Plasminogen.Infection and immunity · 2015Article
- Article
- Mycoplasma pneumoniae, an underutilized model for bacterial cell biology.Journal of bacteriology · 2014Review
- Delineation of immunodominant and cytadherence segment(s) of Mycoplasma pneumoniae P1 gene.BMC microbiology · 2014Article
- Factors that mediate and prevent degradation of the inactive and unstable GudB protein in Bacillus subtilis.Frontiers in microbiology · 2014Article
- Evidence for glycoprotein transport into complex plastids.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- PFunkel: efficient, expansive, user-defined mutagenesis.PloS one · 2012Article
- A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression.PLoS pathogens · 2011Article
- The phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the complete known Ser/Thr kinome suggests the existence of novel kinases.Molecular & cellular proteomics : MCP · 2010Article
- Entry of Spiroplasma citri into Circulifer haematoceps cells involves interaction between spiroplasma phosphoglycerate kinase and leafhopper actin.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Mycoplasma pneumoniae, the UGA opal codon specifies tryptophan rather than a translation stop site. This often makes it difficult to express Mycoplasma proteins in E. coli isolates. In this work, we developed a strategy for the one-step introduction of several mutations. This method, the multiple-mutation reaction, is used to simultaneously replace nine opal codons in the M. pneumoniae glpK gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.