ArticleApplied and environmental microbiology2005
Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp. in water.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 77 citations in OpenAlex.
- Comparison between Legiolert and real time PCR in the detection of Legionella pneumophila from environmental water samples.PloS one · 2025Article
- Biosensing Technologies for DetectingMicroorganisms · 2024Review
- Rapid microbial viability assay using scanning electron microscopy: a proof-of-concept using Phosphotungstic acid staining.Computational and structural biotechnology journal · 2023Article
- A preliminary investigation into bacterial viability using scanning electron microscopy-energy-dispersive X-ray analysis: The case of antibiotics.Frontiers in microbiology · 2022Article
- New Insight regardingMicrobiology spectrum · 2021Article
- A Combined Immunofluorescence and Fluorescent Viability Cocktail Staining Procedure for Rapid Microscopic Detection and Enumeration of Live Legionella pneumophila.Journal of fluorescence · 2021Article
- Prevalence ofIranian journal of medical sciences · 2018Review
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system.Scientific reports · 2017Article
- Determination of viable legionellae in engineered water systems: Do we find what we are looking for?Water research · 2016Review
- Evaluation of Ultrasound-Induced Damage to Escherichia coli and Staphylococcus aureus by Flow Cytometry and Transmission Electron Microscopy.Applied and environmental microbiology · 2016Article
- Contamination of Hospital Water Supplies in Gilan, Iran, with Legionella pneumophila, Escherichia coli, and Pseudomonas aeruginosa.Interdisciplinary perspectives on infectious diseases · 2015Article
- Necessity and effect of combating Legionella pneumophila in municipal shower systems.PloS one · 2014Article
- Detection limits of Legionella pneumophila in environmental samples after co-culture with Acanthamoeba polyphaga.BMC microbiology · 2013Article
- Single-cell analysis of cell viability after a biocide treatment unveils an absence of positive correlation between two commonly used viability markers.MicrobiologyOpen · 2013Article
- The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.PloS one · 2013Article
- Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry.Microbial biotechnology · 2012Article
- Application of EMA-qPCR as a complementary tool for the detection and monitoring of Legionella in different water systems.World journal of microbiology & biotechnology · 2012Article
- Rapid enumeration of active Legionella pneumophila in freshwater environments by the microcolony method combined with direct fluorescent antibody staining.Microbes and environments · 2012Article
- Total and viable Legionella pneumophila cells in hot and natural waters as measured by immunofluorescence-based assays and solid-phase cytometry.Applied and environmental microbiology · 2011Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A sensitive and specific method has been developed to enumerate viable L. pneumophila and other Legionella spp. in water by epifluorescence microscopy in a short period of time (a few hours). This method allows the quantification of L. pneumophila or other Legionella spp. as well as the discrimination between viable and nonviable Legionella. It simultaneously combines the specific detection of Legionella cells using antibodies and a bacterial viability marker (ChemChrome V6), the enumeration being achieved by epifluorescence microscopy. The performance of this immunological double-staining (IDS) method was investigated in 38 natural filterable water samples from different aquatic sources, and the viable Legionella counts were compared with those obtained by the standard culture method. The recovery rate of the IDS method is similar to, or higher than, that of the conventional culture method. Under our experimental conditions, the limit of detection of the IDS method was <176 Legionella cells per liter. The examination of several samples in duplicates for the presence of L. pneumophila and other Legionella spp. indicated that the IDS method exhibits an excellent intralaboratory reproducibility, better than that of the standard culture method. This immunological approach allows rapid measurements in emergency situations, such as monitoring the efficacy of disinfection shock treatments. Although its field of application is as yet limited to filterable waters, the double-staining method may be an interesting alternative (not equivalent) to the conventional standard culture methods for enumerating viable Legionella when rapid detection is required.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.