ArticleApplied and environmental microbiology2005
Evaluation of lateral-flow Clostridium botulinum neurotoxin detection kits for food analysis.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 98 citations in OpenAlex.
- Diagnostic Accuracy of Immunochromatographic Tests for the Detection of Norovirus in Stool Specimens: a Systematic Review and Meta-Analysis.Microbiology spectrum · 2021Pooled it
- Foodborne Botulism Caused byMicroorganisms · 2025Article
- An Advanced Sensing Approach to Biological Toxins with Localized Surface Plasmon Resonance Spectroscopy Based on Their Unique Protein Quaternary Structures.International journal of molecular sciences · 2024Article
- Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin.Sensors (Basel, Switzerland) · 2023Article
- Biodefence research two decades on: worth the investment?The Lancet. Infectious diseases · 2021Review
- Rapid Detection ofInternational journal of environmental research and public health · 2021Article
- Convection-enhanced delivery of botulinum toxin serotype A into the nonhuman primate cisterna magna and hippocampus.Journal of neurosurgery · 2020Article
- Sorbent-incorporated dipstick for direct assaying of proteases.Analytical and bioanalytical chemistry · 2020Article
- Review
- Recommended Immunological Strategies to Screen for Botulinum Neurotoxin-Containing Samples.Toxins · 2015Article
- Article
- Electrophoresis-enhanced detection of deoxyribonucleic acids on a membrane-based lateral flow strip using avian influenza H5 genetic sequence as the model.Sensors (Basel, Switzerland) · 2014Article
- Article
- Interlaboratory and interstudy reproducibility of a novel lateral-flow device and influence of antifungal therapy on detection of invasive pulmonary aspergillosis.Journal of clinical microbiology · 2013Article
- Detection of invasive pulmonary aspergillosis in haematological malignancy patients by using lateral-flow technology.Journal of visualized experiments : JoVE · 2012Article
- A bioanalytical platform for simultaneous detection and quantification of biological toxins.Sensors (Basel, Switzerland) · 2012Article
- Rapid affinity immunochromatography column-based tests for sensitive detection of Clostridium botulinum neurotoxins and Escherichia coli O157.Applied and environmental microbiology · 2010Article
- Multi-wavelength Spatial LED illumination based detector for in vitro detection of Botulinum Neurotoxin A Activity.Sensors and actuators. B, Chemical · 2010Article
- Article
- Lab-on-a-chip for botulinum neurotoxin a (BoNT-A) activity analysis.Lab on a chip · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The suitability and sensitivity of two in vitro lateral-flow assays for detecting Clostridium botulinum neurotoxins (BoNTs) in an assortment of foods were evaluated. Toxin extraction and preparation methods for various liquid, solid, and high-fat-content foods were developed. The lateral-flow assays, one developed by the Naval Medical Research Center (Silver Spring, MD) and the other by Alexeter Technologies (Gaithersburg, MD), are based on the immunodetection of BoNT types A, B, and E. The assays were found to be rapid and easy to perform with minimum requirements for laboratory equipment or skills. They can readily detect 10 ng/ml of BoNT types A and B and 20 ng/ml of BoNT type E. Compared to other in vitro detection methods, these assays are less sensitive, and the assessment of a result is strictly qualitative. However, the assay was found to be simple to use and to require minimal training. The assays successfully detected BoNT types A, B, and E in a wide variety of foods, suggesting their potential usefulness as a preliminary screening system for triaging food samples with elevated BoNT levels in the event of a C. botulinum contamination event.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.