ArticleApplied and environmental microbiology2005
Development of a recA gene-based identification approach for the entire Burkholderia genus.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers, 1 of them a synthesis that pooled it.
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Who cites it
94 citing papers in PubMed, 1 synthesis or guideline pooled it, 224 citations in OpenAlex.
- Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei.PLoS neglected tropical diseases · 2013Pooled it
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- Laboratory strategies for the identification of Burkholderia species: From classical phenotyping to advanced genomic and proteomic approaches.Antonie van Leeuwenhoek · 2026Review
- Quorum sensing in theApplied and environmental microbiology · 2026Review
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- Identifying phenotypic and genetic traits for assessing pathogenic potential and biocontrol capacity in Burkholderia sensu lato strains.The ISME journal · 2026Article
- Genetic Characterization and Symbiotic Performance of Soybean Rhizobia Under Cold and Water-Deficient Conditions in Poland.Plants (Basel, Switzerland) · 2025Article
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- Practical Guidance for Clinical Microbiology Laboratories: Updated guidance for processing respiratory tract samples from people with cystic fibrosis.Clinical microbiology reviews · 2024Review
- A study on the occurrence of Burkholderia cepacia complex in ultrasound gels used in different veterinary clinical settings in India.Veterinary research communications · 2023Article
- Activity of ETX0462 toward SomeAntimicrobial agents and chemotherapy · 2023Article
- Polyyne-producingFrontiers in microbiology · 2023Article
- Genome-based taxonomy of Burkholderia sensu lato: Distinguishing closely related species.Genetics and molecular biology · 2023Article
- Specific biomarker mining and rapid detection ofFrontiers in microbiology · 2023Article
- A Wild Rice RhizobacteriumInternational journal of molecular sciences · 2022Article
- Combined Clinical, Epidemiological, and Genome-Based Analysis Identified a Nationwide Outbreak ofOpen forum infectious diseases · 2022Article
- Molecular Research Comparing the Probabilities ofArchives of Razi Institute · 2022Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia is an important bacterial genus containing species of ecological, biotechnological, and pathogenic interest. With their taxonomy undergoing constant revision and the phenotypic similarity of several species, correct identification of Burkholderia is difficult. A genetic scheme based on the recA gene has greatly enhanced the identification of Burkholderia cepacia complex species. However, the PCR developed for the latter approach was limited by its specificity for the complex. By alignment of existing and novel Burkholderia recA sequences, we designed new PCR primers and evaluated their specificity by testing a representative panel of Burkholderia strains. PCR followed by restriction fragment length polymorphism analysis of an 869-bp portion of the Burkholderia recA gene was not sufficiently discriminatory. Nucleotide sequencing followed by phylogenetic analysis of this recA fragment differentiated both putative and known Burkholderia species and all members of the B. cepacia complex. In addition, it enabled the design of a Burkholderia genus-specific recA PCR that produced a 385-bp amplicon, the sequence of which was also able to discriminate all species examined. Phylogenetic analysis of 188 novel recA genes enabled clarification of the taxonomic position of several important Burkholderia strains and revealed the presence of four novel B. cepacia complex recA lineages. Although the recA phylogeny could not be used as a means to differentiate B. cepacia complex strains recovered from clinical infection versus the natural environment, it did facilitate the identification of clonal strain types of B. cepacia, B. stabilis, and B. ambifaria capable of residing in both niches.
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