ArticleApplied and environmental microbiology2005
Isolation and detection of enterovirus RNA from large-volume water samples by using the NucliSens miniMAG system and real-time nucleic acid sequence-based amplification.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Monitoring of Adenoviruses in Water and Wastewater through Applications of PCR Based Methods.Indian journal of microbiology · 2025Review
- Is an oropharyngeal HPV infection more frequently detectable in women with a genital HPV infection?European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2024Article
- Physiological characteristics, geochemical properties and hydrological variables influencing pathogen migration in subsurface system: What we know or not?Geoscience frontiers · 2022Article
- Article
- Norovirus Outbreak Associated with Swimming in a Recreational Lake Not Influenced by External Human Fecal Sources in The Netherlands, August 2012.International journal of environmental research and public health · 2018Article
- Improved Detection of Norovirus and Hepatitis A Virus in Surface Water by Applying Pre-PCR Processing.Food and environmental virology · 2017Article
- Evaluation of Molecular Methods for the Detection and Quantification of Pathogen-Derived Nucleic Acids in Sediment.Frontiers in microbiology · 2017Article
- Detection of Human Enteric Viruses in Freshwater from European Countries.Food and environmental virology · 2016Article
- A comprehensive method for amplicon-based and metagenomic characterization of viruses, bacteria, and eukaryotes in freshwater samples.Microbiome · 2016Article
- Rapid Detection Strategies for the Global Threat of Zika Virus: Current State, New Hypotheses, and Limitations.Frontiers in microbiology · 2016Review
- Concentration of enteric viruses in large volumes of water using a cartridge-type mixed cellulose ester membrane.Food and environmental virology · 2015Article
- Environmental surveillance of human parechoviruses in sewage in The Netherlands.Applied and environmental microbiology · 2013Article
- Aichi virus in sewage and surface water, the Netherlands.Emerging infectious diseases · 2013Article
- Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines.Virus genes · 2012Article
- Real-time PCR detection of enteric viruses in source water and treated drinking water in Wuhan, China.Current microbiology · 2012Article
- Virus hazards from food, water and other contaminated environments.FEMS microbiology reviews · 2012Review
- Development and comparison of two assay formats for parallel detection of four biothreat pathogens by using suspension microarrays.PloS one · 2012Article
- Achievement V - Methods for breaking the transmission of pathogens along the food chain: Detection of viruses in food.Trends in food science & technology · 2011Article
- Validation of internal controls for extraction and amplification of nucleic acids from enteric viruses in water samples.Applied and environmental microbiology · 2011Article
- Presence of enteric viruses in source waters for drinking water production in The Netherlands.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Concentration of water samples is a prerequisite for the detection of the low virus levels that are present in water and may present a public health hazard. The aim of this study was to develop a rapid, standardized molecular method for the detection of enteroviruses in large-volume surface water samples, using a concentration method suitable for the detection of infectious viruses as well as virus RNA. Concentration of water was achieved by a conventional filter adsorption-elution method and ultrafiltration, resulting in a 10,000-fold concentration of the sample. Isolation of virus RNA by a silica-based RNA extraction method was compared with the nonmagnetic and magnetic NucliSens RNA isolation methods. By using the silica-based RNA extraction method in two out of five samples, enterovirus RNA was detected, whereas four out of five samples were positive following RNA isolation with magnetic silica beads. Moreover, estimated RNA levels increased at least 100 to 500 times. Furthermore, we compared enterovirus detection by an in-house reverse transcription (RT)-PCR with a novel commercially available real-time nucleic acid sequence-based amplification (NASBA) assay. We found that the rapid real-time NASBA assay was slightly less sensitive than our in-house RT-PCR. The advantages, however, of a commercial real-time NASBA assay, like the presence of an internal control RNA, standardization, and enormous decrease in turnaround time, makes it an attractive alternative to RT-PCR.
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