ArticleApplied and environmental microbiology2005
Molecular and biochemical analysis of the galactose phenotype of dairy Streptococcus thermophilus strains reveals four different fermentation profiles.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Exopolysaccharide (EPS)-ProducingFoods (Basel, Switzerland) · 2025Article
- Multi-omics analysis reveals the mechanism for galactose metabolism in mutantCurrent research in food science · 2025Article
- Investigating the Role of β-Disodium Glycerophosphate and Urea in Promoting Growth ofFoods (Basel, Switzerland) · 2024Article
- From genotype to phenotype: computational approaches for inferring microbial traits relevant to the food industry.FEMS microbiology reviews · 2023Review
- Effect of sugar transporter on galactose utilization inFrontiers in microbiology · 2023Article
- Genetic and technological diversity ofFrontiers in microbiology · 2023Article
- Article
- Milk fermentation by monocultures or co-cultures ofFrontiers in bioengineering and biotechnology · 2022Article
- Complete Genome Sequence and Carbohydrates-Active EnZymes (CAZymes) Analysis of Lactobacillus paracasei DTA72, a Potential Probiotic Strain with Strong Capability to Use Inulin.Current microbiology · 2020Article
- Metabolic Profiles of Carbohydrates inFrontiers in microbiology · 2020Article
- Genome Analysis and Physiological Characterization of FourFrontiers in microbiology · 2020Article
- Comparison of gal-lac operons in wild-type galactose-positive and -negative Streptococcus thermophilus by genomics and transcription analysis.Journal of industrial microbiology & biotechnology · 2019Article
- Comparative Genomics ofFrontiers in microbiology · 2019Article
- Differences in Carbohydrates Utilization and Antibiotic Resistance Between Streptococcus macedonicus and Streptococcus thermophilus Strains Isolated from Dairy Products in Italy.Current microbiology · 2018Article
- Comparative Transcriptomic Analysis ofFrontiers in microbiology · 2018Article
- New Insights into Various Production Characteristics of Streptococcus thermophilus Strains.International journal of molecular sciences · 2016Review
- Enhancing the Sweetness of Yoghurt through Metabolic Remodeling of Carbohydrate Metabolism in Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus.Applied and environmental microbiology · 2016Article
- Application of Impedance Microbiology for Evaluating Potential Acidifying Performances of Starter Lactic Acid Bacteria to Employ in Milk Transformation.Frontiers in microbiology · 2016Article
- The art of strain improvement of industrial lactic acid bacteria without the use of recombinant DNA technology.Microbial cell factories · 2014Review
- A selected core microbiome drives the early stages of three popular italian cheese manufactures.PloS one · 2014Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactose-limited fermentations of 49 dairy Streptococcus thermophilus strains revealed four distinct fermentation profiles with respect to galactose consumption after lactose depletion. All the strains excreted galactose into the medium during growth on lactose, except for strain IMDOST40, which also displayed extremely high galactokinase (GalK) activity. Among this strain collection eight galactose-positive phenotypes sensu stricto were found and their fermentation characteristics and Leloir enzyme activities were measured. As the gal promoter seems to play an important role in the galactose phenotype, the galR-galK intergenic region was sequenced for all strains yielding eight different nucleotide sequences (NS1 to NS8). The gal promoter played an important role in the Gal-positive phenotype but did not determine it exclusively. Although GalT and GalE activities were detected for all Gal-positive strains, GalK activity could only be detected for two out of eight Gal-positive strains. This finding suggests that the other six S. thermophilus strains metabolize galactose via an alternative route. For each type of fermentation profile obtained, a representative strain was chosen and four complete Leloir gene clusters were sequenced. It turned out that Gal-positive strains contained more amino acid differences within their gal genes than Gal-negative strains. Finally, the biodiversity regarding lactose-galactose utilization among the different S. thermophilus strains used in this study was shown by RAPD-PCR. Five Gal-positive strains that contain nucleotide sequence NS2 in their galR-galK intergenic region were closely related.
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