ArticleApplied and environmental microbiology2005
OusB, a broad-specificity ABC-type transporter from Erwinia chrysanthemi, mediates uptake of glycine betaine and choline with a high affinity.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Functional and genomic characterization of halotolerant Bacillus paramycoides MSR1 for MICCP-based durability enhancement of cementitious materials under saline conditions.World journal of microbiology & biotechnology · 2026Article
- Limits of a Glycine Betaine-Derived Xenobiotic as a Trojan Horse Antimicrobial.International journal of molecular sciences · 2026Article
- Genome Characterisation of Priestia megaterium mj1212 and Its Synergistic Effect With N-Acetylglucosamine in Enhancing Soybean Salt Stress Tolerance.Plant, cell & environment · 2025Article
- Association Analysis of the Genomic and Functional Characteristics of HalotolerantMicroorganisms · 2025Article
- Genomic Characterization of Probiotic Purple Nonsulfur BacteriaLife (Basel, Switzerland) · 2024Article
- OpuF, a New Bacillus Compatible Solute ABC Transporter with a Substrate-Binding Protein Fused to the Transmembrane Domain.Applied and environmental microbiology · 2018Article
- Review
- Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum.Journal of microbiology (Seoul, Korea) · 2017Article
- Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade.Environmental microbiology · 2015Article
- Ectoine and hydroxyectoine as protectants against osmotic and cold stress: uptake through the SigB-controlled betaine-choline- carnitine transporter-type carrier EctT from Virgibacillus pantothenticus.Journal of bacteriology · 2011Article
- The compatible-solute-binding protein OpuAC from Bacillus subtilis: ligand binding, site-directed mutagenesis, and crystallographic studies.Journal of bacteriology · 2008Article
- Pseudomonas syringae BetT is a low-affinity choline transporter that is responsible for superior osmoprotection by choline over glycine betaine.Journal of bacteriology · 2008Article
- Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function.Journal of bacteriology · 2007Article
- Proline betaine uptake in Sinorhizobium meliloti: Characterization of Prb, an opp-like ABC transporter regulated by both proline betaine and salinity stress.Journal of bacteriology · 2006Article
- The Erwinia chrysanthemi 3937 PhoQ sensor kinase regulates several virulence determinants.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of Erwinia chrysanthemi to cope with environments of elevated osmolality is due in part to the transport and accumulation of osmoprotectants. In this study we have identified a high-affinity glycine betaine and choline transport system in E. chrysanthemi. By using a pool of Tn5-B21 ousA mutants, we isolated a mutant that could grow in the presence of a toxic analogue of glycine betaine (benzyl-glycine betaine) at high osmolalities. This mutant was impaired in its ability to transport all effective osmoprotectants in E. chrysanthemi. The DNA sequence of the regions flanking the transposon insertion site revealed three chromosomal genes (ousVWX) that encode components of an ABC-type transporter (OusB): OusV (ATPase), OusW (permease), and OusX (periplasmic binding protein). The OusB components showed a significant degree of sequence identity to components of ProU from Salmonella enterica serovar Typhimurium and Escherichia coli. OusB was found to restore the uptake of glycine betaine and choline through functional complementation of an E. coli mutant defective in both ProU and ProP osmoprotectant uptake systems. Competition experiments demonstrated that choline, dimethylsulfoniacetate, dimethylsulfoniopropionate, and ectoine were effective competitors for OusB-mediated betaine transport but that carnitine, pipecolate, and proline were not effective. In addition, the analysis of single and double mutants showed that OusA and OusB were the only osmoprotectant transporters operating in E. chrysanthemi.
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