ArticleJournal of clinical microbiology2005
Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridization.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 2 of them syntheses that pooled it.
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Who cites it
42 citing papers in PubMed, 2 syntheses or guidelines pooled it, 132 citations in OpenAlex.
- Prevention, diagnosis, therapy and follow-up care of sepsis: 1st revision of S-2k guidelines of the German Sepsis Society (Deutsche Sepsis-Gesellschaft e.V. (DSG)) and the German Interdisciplinary Association of Intensive Care and Emergency Medicine (Deutsche Interdisziplinäre Vereinigung für Intensiv- und Notfallmedizin (DIVI)).German medical science : GMS e-journal · 2010Guideline
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- Integrated BSI bacteria identifier-on-chip using approximate k-mer matching.Scientific reports · 2026Article
- Diagnostic comparison of microbial culture and polymerase chain reaction for the diagnosis of bacterial meningitis in a tertiary hospital, North West Ethiopia.IJID regions · 2025Article
- Bladder wall-embedded bacteria associated with increased risk of recurrence after electrofulguration in women with antibiotic-recalcitrant urinary tract infection.JU open plus · 2025Article
- Frequency of etiological agents of acute bacterial meningitis using culture and polymerase chain reaction assay.New microbes and new infections · 2021Article
- An improved clinical prediction rule for identifying neonatal bacterial meningitis: a multicenter cohort study.Translational pediatrics · 2021Article
- NOTIFy (non-toxic lyophilized field)-FISH for the identification of biological agents by Fluorescence in situ Hybridization.PloS one · 2020Article
- Isolation and characterization of a potentially novel Siphoviridae phage (vB_SsapS-104) with lytic activity against Staphylococcus saprophyticus isolated from urinary tract infection.Folia microbiologica · 2019Article
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- Rapid and Accurate Diagnosis of Acute Pyogenic Meningitis Due toJournal of clinical and diagnostic research : JCDR · 2017Article
- Peptide Nucleic Acid-Fluorescence In Situ Hybridization for Detection of Staphylococci From Endophthalmitis Isolates: A Proof-of-Concept Study.Investigative ophthalmology & visual science · 2017Article
- Ventilator-Associated Pneumonia: The Role of Emerging Diagnostic Technologies.Seminars in respiratory and critical care medicine · 2017Review
- Usefulness of inflammatory biomarkers in discriminating between bacterial and aseptic meningitis in hospitalized children from a population with low vaccination coverage.Archives of medical science : AMS · 2016Article
- Review
- Direct identification of major Gram-negative pathogens in respiratory specimens by respiFISH® HAP Gram (-) Panel, a beacon-based FISH methodology.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Epidemiology and Risk Factors Associated with Developing Bacterial Meningitis among Children in Gaza Strip.Iranian journal of public health · 2014Article
- A versatile PDMS/paper hybrid microfluidic platform for sensitive infectious disease diagnosis.Analytical chemistry · 2014Article
- Beacon-based (bbFISH®) technology for rapid pathogens identification in blood cultures.BMC microbiology · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time PCR and fluorescence in situ hybridization (FISH) were evaluated as rapid methods for the diagnosis of bacterial meningitis and compared to standard diagnostic procedures. For PCR, a LightCycler approach was chosen, implementing eubacterial and specific PCR assays for the most relevant bacteria. For FISH, a similar probe set containing eubacterial and specific probes was composed of published and newly designed probes. Both methods were evaluated by use of cerebrospinal fluid (CSF) samples from patients with suspected bacterial meningitis. For all microscopy- and culture-positive samples (n = 28), the eubacterial PCR was positive. In addition, all identifiable pathogens were detected with specific PCR assays, according to an algorithm based on the Gram stain. The FISH method detected the pathogen in 13 of 18 positive samples. While the FISH method remained negative for all microscopy- and culture-negative samples (n = 113), the eubacterial PCR was positive for five of these samples. Sequencing of the amplicon revealed the presence of Neisseria meningitidis, Streptococcus agalactiae, and Haemophilus influenzae in three of these five samples. In addition, samples with discordant results by culture and microscopy were successfully investigated by PCR (10 samples) and FISH (5 samples). In conclusion, PCR is a highly sensitive tool for rapid diagnosis of bacterial meningitis. FISH is less sensitive but is useful for the identification of CSF samples showing bacteria in the Gram stain. Based on our results, an approach for laboratory diagnosis of meningitis including PCR and FISH is discussed.
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