Evidence map›Paper›PMID 16000459›Full record

ArticleJournal of clinical microbiology2005

Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates.

Patrice Francois, Antoine Huyghe, Yvan Charbonnier, Manuela Bento, Sébastien Herzig, Ivan Topolski, Bénédicte Fleury, Daniel Lew, Pierre Vaudaux, Stephan Harbarth and 5 more

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
4.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 103 citations in OpenAlex.

  1. Mortality of CC398 Staphylococcus aureus bloodstream infections among hospitalised patients in a Swiss university hospital: a retrospective cohort study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Article
  2. Article
  3. Marked Presence of Methicillin-ResistantAnimals : an open access journal from MDPI · 2020
    Article
  4. Article
  5. Antimicrobial activity of ceftaroline against methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 2013-2014 at the Geneva University Hospitals.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017
    Article
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  11. Prevalence of isolates with reduced glycopeptide susceptibility in orthopedic device-related infections due to methicillin-resistant Staphylococcus aureus.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012
    Article
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  14. Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 4 countries.

Patrice FrancoisGenomic Research Laboratory, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland. patrice.francois@genomic.ch
Antoine Huyghe
Yvan Charbonnier
Manuela Bento
Sébastien Herzig
Ivan Topolski
Bénédicte Fleury
Daniel Lew
Pierre Vaudaux
Stephan Harbarth
Willem van Leeuwen
Alex van Belkum
Dominique S Blanc
Didier Pittet
Jacques Schrenzel
Center for Genomic Science · ITOffice of Infectious Diseases · USErasmus MC · NLUniversity Hospital of Lausanne · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fast and reliable genotyping methods that allow real-time epidemiological surveillance would be instrumental to monitoring of the spread of methicillin-resistant Staphylococcus aureus. We describe an automated variable-number tandem repeat-based method for the rapid genotyping of Staphylococcus aureus. Multiplex PCR amplifications with eight primer pairs that target gene regions with variable numbers of tandem repeats were resolved by microcapillary electrophoresis and automatically assessed by cluster analysis. This genotyping technique was evaluated for its discriminatory power and reproducibility with clinical isolates of various origins, including a panel of control strains previously characterized by several typing methods and collections from either long-term carriers or defined nosocomial outbreaks. All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time.

Indexed as

Bacterial Typing TechniquesAutomationBacterial ProteinsDisease OutbreaksElectrophoresis, Gel, Pulsed-FieldGenotypeHumansMethicillin ResistanceMinisatellite RepeatsPolymerase Chain ReactionReproducibility of ResultsStaphylococcal InfectionsStaphylococcus aureusTime FactorsBacterial Proteins

Identifiers

PMID16000459
PMCPMC1169139
OpenAlexW1998816689

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.