ArticleJournal of clinical microbiology2005
Multilocus DNA sequence comparisons rapidly identify pathogenic molds.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 131 citations in OpenAlex.
- A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species.PloS one · 2014Trial
- New strategy for rapid diagnosis and characterization of fungal infections: the example of corneal scrapings.PloS one · 2012Trial
- A broad-range polymerase chain reaction on a formalin-fixed, paraffin-embedded tissue is a powerful diagnostic tool, but requires cautious interpretation: a case report.Frontiers in medicine · 2026Article
- Non-fungal pathogens detected by broad-range fungal polymerase chain reaction.Journal of clinical microbiology · 2025Article
- Broad-range polymerase chain reaction and sequencing for the diagnosis of infectious diseases.Microbiology spectrum · 2025Article
- A retrospective observational study of mNGS test utilization to examine the role of diagnostic stewardship at two academic medical centers.Journal of clinical microbiology · 2024Observational
- Successful Treatment of Balamuthia mandrillaris Granulomatous Amebic Encephalitis with Nitroxoline.Emerging infectious diseases · 2023Article
- Exploring diversity rock-inhabiting fungi from northern Thailand: a new genus and three new species belonged to the familyFrontiers in cellular and infection microbiology · 2023Article
- Nucleic Acid-Based Detection ofJournal of fungi (Basel, Switzerland) · 2022Review
- Cokeromyces recurvatus Incidentally Found in a Patient with Gastric Outlet Obstruction.Mycopathologia · 2022Article
- Rapid and Accurate Detection ofPathogens (Basel, Switzerland) · 2022Article
- Species Diversity, Distribution, and Phylogeny ofJournal of fungi (Basel, Switzerland) · 2022Article
- High Clinical Impact of Broad-Range Fungal PCR in Suspected Fungal Sinusitis.Journal of clinical microbiology · 2021Article
- A Disfiguring Rash.Open forum infectious diseases · 2021Article
- Diversity of microbiota in Slovak summer ewes' cheese "Bryndza".Open life sciences · 2021Article
- Clinical Utility of Universal Broad-Range Polymerase Chain Reaction Amplicon Sequencing for Pathogen Identification: A Retrospective Cohort Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Article
- Isavuconazole Prophylaxis in Patients With Hematologic Malignancies and Hematopoietic Cell Transplant Recipients.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Article
- Article
- A Novel Species ofFrontiers in cellular and infection microbiology · 2020Article
- Microbial Identification Using DNA Target Amplification and Sequencing: Clinical Utility and Impact on Patient Management.Open forum infectious diseases · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The increasing incidence of opportunistic fungal infections necessitates rapid and accurate identification of the associated fungi to facilitate optimal patient treatment. Traditional phenotype-based identification methods utilized in clinical laboratories rely on the production and recognition of reproductive structures, making identification difficult or impossible when these structures are not observed. We hypothesized that DNA sequence analysis of multiple loci is useful for rapidly identifying medically important molds. Our study included the analysis of the D1/D2 hypervariable region of the 28S ribosomal gene and the internal transcribed spacer (ITS) regions 1 and 2 of the rRNA operon. Two hundred one strains, including 143 clinical isolates and 58 reference and type strains, representing 43 recognized species and one possible new species, were examined. We generated a phenotypically validated database of 118 diagnostic alleles. DNA length polymorphisms detected among ITS1 and ITS2 PCR products can differentiate 20 of 33 species of molds tested, and ITS DNA sequence analysis permits identification of all species tested. For 42 of 44 species tested, conspecific strains displayed >99% sequence identity at ITS1 and ITS2; sequevars were detected in two species. For all 44 species, identifications by genotypic and traditional phenotypic methods were 100% concordant. Because dendrograms based on ITS sequence analysis are similar in topology to 28S-based trees, we conclude that ITS sequences provide phylogenetically valid information and can be utilized to identify clinically important molds. Additionally, this phenotypically validated database of ITS sequences will be useful for identifying new species of pathogenic molds.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.