ArticleJournal of clinical microbiology2005
Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00452153 (Evaluation of Legionella PCR Techniques for the Routine Diagnosis of Legionellosis), which is not on this map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Evaluation of Legionella PCR Techniques for the Routine Diagnosis of Legionellosis
Who cites it
22 citing papers in PubMed, 64 citations in OpenAlex.
- Development of a DNA microarray assay for rapid detection of fifteen bacterial pathogens in pneumonia.BMC microbiology · 2020Article
- Mycoplasma pneumoniae from the Respiratory Tract and Beyond.Clinical microbiology reviews · 2017Review
- Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria.Journal of negative results in biomedicine · 2016Article
- The role of multiplex PCR test in identification of bacterial pathogens in lower respiratory tract infections.Pakistan journal of medical sciences · 2014Article
- Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2014Article
- Detection of multiple viral and bacterial infections in acute exacerbation of chronic obstructive pulmonary disease: a pilot prospective study.Journal of medical virology · 2013Article
- Comparative evaluation of six commercialized multiplex PCR kits for the diagnosis of respiratory infections.PloS one · 2013Article
- Molecular methods for the detection of Mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology.The Journal of molecular diagnostics : JMD · 2012Review
- Reply to "how to diagnose Mycoplasma pneumoniae etiology in a child with pneumonia".European journal of pediatrics · 2012Article
- Detection of Mycoplasma pneumoniae in different respiratory specimens.European journal of pediatrics · 2011Article
- Reversible neurological syndromes with atypical pneumonia.Annals of Indian Academy of Neurology · 2011Article
- Community-acquired pneumonia due to Legionella pneumophila, the utility of PCR, and a review of the antibiotics used.International journal of general medicine · 2011Article
- Article
- Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Review
- Evaluation of a nested-PCR-derived sequence-based typing method applied directly to respiratory samples from patients with Legionnaires' disease.Journal of clinical microbiology · 2009Article
- Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens.Annals of clinical microbiology and antimicrobials · 2009Article
- [Microbiological diagnosis: Etiologic driven and syndrome driven].Antibiotiques (Paris, France : 1999) · 2009Article
- Molecular diagnostics for detection of bacterial and viral pathogens in community-acquired pneumonia.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2008Review
- Comparison and evaluation of four commercial kits relative to an in-house immunofluorescence test for detection of antibodies against Legionella pneumophila.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2008Article
- Utility of real-time PCR for diagnosis of Legionnaires' disease in routine clinical practice.Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study describes the development and evaluation of a new commercial test, Chlamylege (Argene Inc.), which allows the simultaneous detection in respiratory samples of Chlamydophila pneumoniae, Mycoplasma pneumoniae, and most Legionella species, as well as PCR inhibitors, by using a multiplex PCR and microplate hybridization. The sensitivities of Chlamylege were 1 x 10(-3) IFU, 5 x 10(-2) color-changing units, and 1 CFU per reaction tube for C. pneumoniae, M. pneumoniae, and Legionella pneumophila, respectively. A cohort of 154 clinical samples from patients with documented respiratory infections was analyzed by the kit, including 2 samples from patients with C. pneumoniae infection, 9 samples from patients with M. pneumoniae infection, 19 samples from patients with Legionella species infection, and 114 samples that tested negative for the three pathogens. All the positive specimens were correctly detected and identified by the Chlamylege kit, and no false-positive result was observed with the negative samples. The kit was then evaluated in a pediatric prospective study that included 220 endotracheal aspirates, and the results were compared with those obtained by three single in-house PCR assays. Four specimens were found to be positive for C. pneumoniae and six were found to be positive for M. pneumoniae by using both strategies. The Chlamylege kit detected two additional samples positive for M. pneumoniae and one additional sample positive for a Legionella species other than L. pneumophila; these three samples were shown to be true positive by other techniques. These overall results demonstrate that the Chlamylege assay is sensitive, specific, and convenient for the rapid detection and identification of atypical pathogens in clinical samples from patients with respiratory infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.