ArticleProceedings of the National Academy of Sciences of the United States of America2005
The binding of the ubiquitous transcription factor Sp1 at the locus control region represses the expression of beta-like globin genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- In vivo genome editing of human haematopoietic stem cells for treatment of blood disorders using mRNA delivery.Nature biomedical engineering · 2026Article
- miRNA-Mediated Regulation of γ-Globin to β-Globin Switching: Therapeutic Potential in β-Hemoglobinopathies.International journal of molecular sciences · 2026Review
- Hemogen/BRG1 cooperativity modulates promoter and enhancer activation during erythropoiesis.Blood · 2022Article
- Yolk sac, but not hematopoietic stem cell-derived progenitors, sustain erythropoiesis throughout murine embryonic life.The Journal of experimental medicine · 2021Article
- Nuclear Fibroblast Growth Factor Receptor Signaling in Skeletal Development and Disease.Current osteoporosis reports · 2019Review
- Review
- Association study of sepiapterin reductase gene promoter polymorphisms with schizophrenia in a Han Chinese population.Neuropsychiatric disease and treatment · 2015Article
- A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.Molecular and cellular biology · 2013Article
- Human Vav1 expression in hematopoietic and cancer cell lines is regulated by c-Myb and by CpG methylation.PloS one · 2012Article
- Article
- Epigenetic modulation of the protein kinase A RIIα (PRKAR2A) gene by histone deacetylases 1 and 2 in human smooth muscle cells.Journal of cellular and molecular medicine · 2011Article
- Article
- A role for Sp1 in transcriptional regulation of phosphatidylethanolamine N-methyltransferase in liver and 3T3-L1 adipocytes.The Journal of biological chemistry · 2010Article
- Role of the BCA2 ubiquitin E3 ligase in hormone responsive breast cancer.The open cancer journal · 2010Article
- Integrative approaches to the prediction of protein functions based on the feature selection.BMC bioinformatics · 2009Article
- Locus control region mediated regulation of adult beta-globin gene expression.Journal of cellular biochemistry · 2008Article
- Promoter variant in the GRK3 gene associated with bipolar disorder alters gene expression.Biological psychiatry · 2008Article
- Kruppel-like Factors (KLFs) in muscle biology.Journal of molecular and cellular cardiology · 2007Review
- Lineage-specific activators affect beta-globin locus chromatin in multipotent hematopoietic progenitors.The EMBO journal · 2006Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the function of transcription factor Sp1 in beta-like globin gene activation, we analyzed the recruitment of Sp1, fetal Krüppel-like factor 2 (FKLF2), and related factors at the human beta-globin locus in a human fetal liver and mouse erythroleukemia hybrid cell (A181gamma cell) that contains a single copy of human chromosome 11. Sp1 binds at the GT boxes of the cis-elements throughout the beta-locus, but it is phosphorylated and lost over DNase I hypersensitive site (HS)2, HS3, HS4, and the human beta-globin gene promoter after A181gamma cell differentiation. The binding of FKLF2 at HS2 and HS3 was unchanged. Histone deacetylase 1, which could be recruited by Sp1, is also lost over HS2 and HS3 after differentiation, resulting in the acetylation of histones 3 and 4 across the human beta-globin locus. We previously detected in vivo GT footprints over the beta-globin locus after A181gamma differentiation. Here, we report that after differentiation, the p300/CREB-binding protein-associated factor is recruited by FKLF2 to the locus control region to acetylate histones 3 and 4 at the human beta-globin gene locus. Our results suggest that Sp1 is an inhibitor of beta-like globin gene transcription during erythroid terminal differentiation. Its phosphorylation and release allow the erythroid-specific FKLF2 or erythroid Krüppel-like factor to interact with other erythroid-specific transcription factors to initiate the transcription of beta-like globin genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.