ArticleGenetics2005
Genome-wide synthetic lethal screens identify an interaction between the nuclear envelope protein, Apq12p, and the kinetochore in Saccharomyces cerevisiae.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- RNA Polymerase III Promoters Compatible with CRISPR Gene Regulation inACS synthetic biology · 2025Article
- Parameter Estimation and Variable Selection for Big Systems of Linear Ordinary Differential Equations: A Matrix-Based Approach.Journal of the American Statistical Association · 2019Article
- Article
- Interactions between the kinetochore complex and the protein kinase A pathway in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2012Article
- KNL1/Spc105 recruits PP1 to silence the spindle assembly checkpoint.Current biology : CB · 2011Article
- Ybp2 associates with the central kinetochore of Saccharomyces cerevisiae and mediates proper mitotic progression.PloS one · 2008Article
- Synthetic lethal interactions identify phenotypic "interologs" of the spindle assembly checkpoint components.Genetics · 2007Article
- The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes.The Journal of cell biology · 2007Article
- Spc24 and Stu2 promote spindle integrity when DNA replication is stalled.Molecular biology of the cell · 2007Article
- Nuclear envelope insertion of spindle pole bodies and nuclear pore complexes.Nucleus (Austin, Tex.)Review
- Integrating complex functions: coordination of nuclear pore complex assembly and membrane expansion of the nuclear envelope requires a family of integral membrane proteins.Nucleus (Austin, Tex.)Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maintenance of genome stability is a fundamental requirement for normal cell cycle progression. The budding yeast Saccharomyces cerevisiae is an excellent model to study chromosome maintenance due to its well-defined centromere and kinetochore, the region of the chromosome and associated protein complex, respectively, that link chromosomes to microtubules. To identify genes that are linked to chromosome stability, we performed genome-wide synthetic lethal screens using a series of novel temperature-sensitive mutations in genes encoding a central and outer kinetochore protein. By performing the screens using different mutant alleles of each gene, we aimed to identify genetic interactions that revealed diverse pathways affecting chromosome stability. Our study, which is the first example of genome-wide synthetic lethal screening with multiple alleles of a single gene, demonstrates that functionally distinct mutants uncover different cellular processes required for chromosome maintenance. Two of our screens identified APQ12, which encodes a nuclear envelope protein that is required for proper nucleocytoplasmic transport of mRNA. We find that apq12 mutants are delayed in anaphase, rereplicate their DNA, and rebud prior to completion of cytokinesis, suggesting a defect in controlling mitotic progression. Our analysis reveals a novel relationship between nucleocytoplasmic transport and chromosome stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.