ArticleBMC infectious diseases2005
Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method.
Article in BMC infectious diseases, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 66 citations in OpenAlex.
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- Recent Developments in Nanotechnology-Based Biosensors for the Diagnosis of Coronavirus.Plasmonics (Norwell, Mass.) · 2023Review
- Developments in the HCV Screening Technologies Based on the Detection of Antigens and Antibodies.Sensors (Basel, Switzerland) · 2019Review
- Graphene Oxide-Based Nanostructured DNA Sensor.Biosensors · 2019Article
- Applications of gold nanoparticles in virus detection.Theranostics · 2018Review
- Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection.Viruses · 2017Review
- Application of nanodiagnostics in point-of-care tests for infectious diseases.International journal of nanomedicine · 2017Review
- Role of metal and metal oxide nanoparticles as diagnostic and therapeutic tools for highly prevalent viral infections.Nanomedicine : nanotechnology, biology, and medicine · 2017Review
- Advanced Nanobiomaterials: Vaccines, Diagnosis and Treatment of Infectious Diseases.Molecules (Basel, Switzerland) · 2016Review
- Detection of antibodies against avian influenza virus by protein microarray using nucleoprotein expressed in insect cells.The Journal of veterinary medical science · 2015Article
- Evidence-based consensus on the diagnosis, prevention and management of hepatitis C virus disease.World journal of hepatology · 2015Review
- A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA.Nanoscale research letters · 2011Article
- Nanobioimaging and sensing of infectious diseases.Advanced drug delivery reviews · 2010Review
- Human body fluid proteome analysis.Proteomics · 2006Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundViral hepatitis due to hepatitis B virus and hepatitis C virus are major public health problems all over the world. Traditional detection methods including polymerase chain reaction (PCR)-based assays and enzyme-linked immunosorbent assays (ELISA) are expensive and time-consuming. In our assay, a protein chip assay using Nano-gold Immunological Amplification and Silver Staining (NIASS) method was applied to detect HBV and HCV antibodies rapidly and simultaneously.
methodsChemically modified glass slides were used as solid supports (named chip), on which several antigens, including HBsAg, HBeAg, HBcAg and HCVAg (a mixture of NS3, NS5 and core antigens) were immobilized respectively. Colloidal nano-gold labelled staphylococcal protein A (SPA) was used as an indicator and immunogold silver staining enhancement technique was applied to amplify the detection signals, producing black image on array spots, which were visible with naked eyes. To determine the detection limit of the protein chip assay, a set of model arrays in which human IgG was spotted were structured and the model arrays were incubated with different concentrations of anti-IgG. A total of 305 serum samples previously characterized with commercial ELISA were divided into 4 groups and tested in this assay.
resultsWe prepared mono-dispersed, spherical nano-gold particles with an average diameter of 15 +/- 2 nm. Colloidal nano-gold-SPA particles observed by TEM were well-distributed, maintaining uniform and stable. The optimum silver enhancement time ranged from 8 to 12 minutes. In our assay, the protein chips could detect serum antibodies against HBsAg, HBeAg, HBcAg and HCVAg with the absence of the cross reaction. In the model arrays, the anti-IgG as low as 3 ng/ml could be detected. The data for comparing the protein chip assay with ELISA indicated that no distinct difference (P > 0.05) existed between the results determined by our assay and ELISA respectively.
conclusionResults showed that our assay can be applied with serology for the detection of HBV and HCV antibodies rapidly and simultaneously in clinical detection.
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