ArticleMicrobial cell factories2005
Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones.
Article in Microbial cell factories, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Further characterization and engineering of an 11-amino acid motif for enhancing recombinant soluble protein expression.Microbial cell factories · 2025Article
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- Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide.Journal of microbiology (Seoul, Korea) · 2022Article
- Small-Molecule Gankyrin Inhibition as a Therapeutic Strategy for Breast and Lung Cancer.Journal of medicinal chemistry · 2022Article
- Identification of a covalent binder to the oncoprotein gankyrin using a NIR-Based OBOC screening method.RSC advances · 2021Article
- Enhanced Production of the Mical Redox Domain for Enzymology and F-actin Disassembly Assays.International journal of molecular sciences · 2021Article
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- β-Catenin signaling dynamics regulate cell fate in differentiating neural stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Mammalian Expression andACS omega · 2020Article
- VPS37A directs ESCRT recruitment for phagophore closure.The Journal of cell biology · 2019Article
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- Switching Cyclic Nucleotide-Selective Activation of Cyclic Adenosine Monophosphate-Dependent Protein Kinase Holoenzyme Reveals Distinct Roles of Tandem Cyclic Nucleotide-Binding Domains.ACS chemical biology · 2017Article
- Mutations of PKA cyclic nucleotide-binding domains reveal novel aspects of cyclic nucleotide selectivity.The Biochemical journal · 2017Article
- TSTMP: target selection for structural genomics of human transmembrane proteins.Nucleic acids research · 2017Article
- Crystal Structure of PKG I:cGMP Complex Reveals a cGMP-Mediated Dimeric Interface that Facilitates cGMP-Induced Activation.Structure (London, England : 1993) · 2016Article
- Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets human proteins for structure determination. It has implemented high throughput procedures for all steps from cloning to structure calculation. This article describes the selection of human target proteins for structure analysis, our high throughput cloning strategy, and the expression of human proteins in Escherichia coli host cells. RESULTS AND
conclusionProtein expression and sequence data of 1414 E. coli expression clones representing 537 different proteins are presented. 139 human proteins (18%) could be expressed and purified in soluble form and with the expected size. All E. coli expression clones are publicly available to facilitate further functional characterisation of this set of human proteins.
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