Evidence map›Paper›PMID 15996681›Full record

ArticleJournal of immunological methods2005

Myoglobin immunoassay based on metal particle-enhanced fluorescence.

Evgenia G Matveeva, Zygmunt Gryczynski, Joseph R Lakowicz

Open access · greenAbstract read
In one paragraph

Article in Journal of immunological methods, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 84 citations in OpenAlex.

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  16. Near-infrared squaraine dyes for fluorescence enhanced surface assay.Dyes and pigments : an international journal · 2009
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Evgenia G MatveevaCenter for Fluorescence Spectroscopy, University of Maryland at Baltimore Medical School, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA. eva@cfs.umbi.umd.edu
Zygmunt Gryczynski
Joseph R Lakowicz
University of Maryland, Baltimore · US

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006
$7.4M
Fluorophore-Metallic Colloid Conjugates for Medical ImagR33EB001690 · NIBIB · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI GRYCZYNSKI, ZYGMUNT · 2005 to 2006
$304k
Fluorophore-Metallic Colloid Conjugates for Medical ImagR21EB001690 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI GRYCZYNSKI, ZYGMUNT · 2003 to 2003
$121k
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-08119NIBIB NIH HHS EB-1690NIBIB NIH HHS R21 EB001690NIBIB NIH HHS R33 EB001690
6 · The paper itself

Abstract

Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10-15-fold if compared to the signal from the glass substrate not modified with a SIF. A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection.

Indexed as

Antibodies, MonoclonalBiomarkersFluorescent DyesFluoroimmunoassayKineticsMyoglobinProtein BindingRhodaminesStaining and LabelingAntibodies, MonoclonalBiomarkersFluorescent DyesMyoglobinRhodamines

Identifiers

PMID15996681
PMCPMC6816259
OpenAlexW2028175192

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.