ArticleHistochemistry and cell biology2005
Improvement on the visualization of cytoskeletal structures of protozoan parasites using high-resolution field emission scanning electron microscopy (FESEM).
Article in Histochemistry and cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 57 citations in OpenAlex.
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- Oral Infection and Survival of Trypanosoma cruzi in Sugarcane Juice Conditioned at Different Temperatures.Acta parasitologica · 2024Article
- Nanoarchitecture of the ventral disc of Giardia intestinalis as revealed by high-resolution scanning electron microscopy and helium ion microscopy.Histochemistry and cell biology · 2022Article
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- An overview on kinetoplastid paraflagellar rod.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2015Review
- An historical perspective on how advances in microscopic imaging contributed to understanding the Leishmania Spp. and Trypanosoma cruzi host-parasite relationship.BioMed research international · 2014Review
- Extracellular amastigotes of Trypanosoma cruzi are potent inducers of phagocytosis in mammalian cells.Cellular microbiology · 2013Article
- Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei.Journal of structural biology · 2012Article
- The Trypanosoma brucei AIR9-like protein is cytoskeleton-associated and is required for nucleus positioning and accurate cleavage furrow placement.Molecular microbiology · 2012Article
- Proteomic analysis of the ventral disc of Giardia lamblia.BMC research notes · 2012Article
- Heterogeneity in the sensitivity of microtubules of Giardia lamblia to the herbicide oryzalin.Parasitology research · 2010Article
- The paraflagellar rod of kinetoplastid parasites: from structure to components and function.International journal for parasitology · 2010Review
- Structure and elastic properties of tunneling nanotubes.European biophysics journal : EBJ · 2008Article
- The histochemistry and cell biology vade mecum: a review of 2005-2006.Histochemistry and cell biology · 2006Review
- Recent progress in histochemistry and cell biology: the state of the art 2005.Histochemistry and cell biology · 2005Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The association of high resolution field emission scanning electron microscopy (FESEM), with a more efficient system of secondary electron (SE) collection and in-lens specimen position, provided a great improvement in the specimen's topographical contrast and in the generation of high-resolution images. In addition, images obtained with the use of the high-resolution backscattered electrons (BSE) detector provided a powerful tool for immunocytochemical analysis of biological material. In this work, we show the contribution of the FESEM to the detailed description of cytoskeletal structures of the protozoan parasites Herpetomonas megaseliae, Trypanosoma brucei and Giardia lamblia. High-resolution images of detergent extracted H. megaseliae and T. brucei showed the profile of the cortical microtubules, also known as sub-pellicular microtubules (SPMT), and protein bridges cross-linking them. Also, it was possible to visualize fine details of the filaments that form the lattice-like structure of the paraflagellar rod (PFR) and its connection with the axoneme. In G. lamblia, it was possible to observe the intricate structure of the adhesive disk, funis (a microtubular array) and other cytoskeletal structures poorly described previously. Since most of the stable cytoskeletal structures of this protozoan rely on tubulin, we used the BSE images to accurately map immunolabeled tubulin in its cytoskeleton. Our results suggest that the observation of detergent extracted parasites using FESEM associated to backscattered analysis of immunolabeled specimens represents a new approach for the study of parasite cytoskeletal elements and their protein associations.
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