ArticleJournal of bacteriology2005
Rapid detection of colicin E9-induced DNA damage using Escherichia coli cells carrying SOS promoter-lux fusions.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- Heterogeneity in the spontaneous induction of the promoter of the ColE9 operon in Escherichia coli.Archives of microbiology · 2022Article
- Porin threading drives receptor disengagement and establishes active colicin transport through Escherichia coli OmpF.The EMBO journal · 2021Article
- Intermembrane crosstalk drives inner-membrane protein organization in Escherichia coli.Nature communications · 2018Article
- Evolution of Bacterial Pathogens Within the Human Host.Microbiology spectrum · 2016Review
- Detection of induced synthesis of colicin E9 using ColE9p::gfpmut2 based reporter system.World journal of microbiology & biotechnology · 2014Article
- Immunity protein release from a cell-bound nuclease colicin complex requires global conformational rearrangement.MicrobiologyOpen · 2013Article
- A force-activated trip switch triggers rapid dissociation of a colicin from its immunity protein.PLoS biology · 2013Article
- Conformational properties of the unfolded state of Im7 in nondenaturing conditions.Journal of molecular biology · 2012Article
- Energy-dependent immunity protein release during tol-dependent nuclease colicin translocation.The Journal of biological chemistry · 2009Article
- The role of SOS boxes in enteric bacteriocin regulation.Microbiology (Reading, England) · 2008Article
- Investigating early events in receptor binding and translocation of colicin E9 using synchronized cell killing and proteolytic cleavage.Journal of bacteriology · 2008Article
- Colicin E2 is still in contact with its receptor and import machinery when its nuclease domain enters the cytoplasm.Journal of bacteriology · 2007Article
- Colicin biology.Microbiology and molecular biology reviews : MMBR · 2007Review
- Release of immunity protein requires functional endonuclease colicin import machinery.Journal of bacteriology · 2006Article
- Interactions of TolB with the translocation domain of colicin E9 require an extended TolB box.Journal of bacteriology · 2005Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ColE9 is a plasmid-encoded protein antibiotic produced by Escherichia coli and closely related species that kills E. coli cells expressing the BtuB receptor. The 15-kDa cytotoxic DNase domain of colicin E9 preferentially nicks double-stranded DNA at thymine bases and shares a common active-site structural motif with a variety of other nucleases, including the H-N-H homing endonucleases and the apoptotic CAD proteins of eukaryotes. Studies of the mechanism by which the DNase domain of ColE9 reaches the cytoplasm of E. coli cells are limited by the lack of a rapid, sensitive assay for the DNA damage that results. Here, we report the development of an SOS promoter-lux fusion reporter system for monitoring DNA damage in colicin-treated cells and illustrate the value of this reporter system in experiments that probe the mechanism and time required for the DNase domain of colicin E9 to reach the cytoplasm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.