ArticleJournal of bacteriology2005
Addition of poly(A) and heteropolymeric 3' ends in Bacillus subtilis wild-type and polynucleotide phosphorylase-deficient strains.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Oligo(uridine) tailing and exonucleolytic decay drive 40S ribosome degradation in response to stress.Molecular cell · 2026Article
- Distribution of poly(A) polymerase I in bacteria: an expanded role for horizontal gene transfer.Microbiology (Reading, England) · 2026Article
- Article
- Acquisition ofMicrobial genomics · 2021Article
- RNase J1 and J2 Are Host-Encoded Factors for Plasmid Replication.Frontiers in microbiology · 2021Article
- Regulation of mRNA Stability During Bacterial Stress Responses.Frontiers in microbiology · 2020Review
- Phylogeny and Evolution of RNA 3'-Nucleotidyltransferases in Bacteria.Journal of molecular evolution · 2019Article
- Analysis of tRNABrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- New Insights into the Relationship between tRNA Processing and Polyadenylation in Escherichia coli.Trends in genetics : TIG · 2019Review
- RNA polyadenylation and its consequences in prokaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Mapping of internal monophosphate 5' ends of Bacillus subtilis messenger RNAs and ribosomal RNAs in wild-type and ribonuclease-mutant strains.Nucleic acids research · 2016Article
- Expression of multiple Bacillus subtilis genes is controlled by decay of slrA mRNA from Rho-dependent 3' ends.Nucleic acids research · 2016Article
- Initiation of mRNA decay in bacteria.Cellular and molecular life sciences : CMLS · 2014Review
- Licensing and due process in the turnover of bacterial RNA.RNA biology · 2013Review
- Three essential ribonucleases-RNase Y, J1, and III-control the abundance of a majority of Bacillus subtilis mRNAs.PLoS genetics · 2012Article
- Polynucleotide phosphorylase exonuclease and polymerase activities on single-stranded DNA ends are modulated by RecN, SsbA and RecA proteins.Nucleic acids research · 2011Article
- Bacillus subtilis polynucleotide phosphorylase 3'-to-5' DNase activity is involved in DNA repair.Nucleic acids research · 2009Article
- Geobacter sulfurreducens contains separate C- and A-adding tRNA nucleotidyltransferases and a poly(A) polymerase.Journal of bacteriology · 2009Article
- Identification of new noncoding RNAs in Listeria monocytogenes and prediction of mRNA targets.Nucleic acids research · 2007Article
- Polyadenylation of ribosomal RNA in human cells.Nucleic acids research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Polyadenylation plays a role in decay of some bacterial mRNAs, as well as in the quality control of stable RNA. In Escherichia coli, poly(A) polymerase I (PAP I) is the main polyadenylating enzyme, but the addition of 3' tails also occurs in the absence of PAP I via the synthetic activity of polynucleotide phosphorylase (PNPase). The nature of 3'-tail addition in Bacillus subtilis, which lacks an identifiable PAP I homologue, was studied. Sizing of poly(A) sequences revealed a similar pattern in wild-type and PNPase-deficient strains. Sequencing of 152 cloned cDNAs, representing 3'-end sequences of nontranslated and translated RNAs, revealed modified ends mostly on incomplete transcripts, which are likely to be decay intermediates. The 3'-end additions consisted of either short poly(A) sequences or longer heteropolymeric ends with a mean size of about 40 nucleotides. Interestingly, multiple independent clones exhibited complex heteropolymeric ends of very similar but not identical nucleotide sequences. Similar polyadenylated and heteropolymeric ends were observed at 3' ends of RNA isolated from wild-type and pnpA mutant strains. These data demonstrated that, unlike the case of some other bacterial species and chloroplasts, PNPase of Bacillus subtilis is not the major enzyme responsible for the addition of nucleotides to RNA 3' ends.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.