ArticlePlant molecular biology2005
Translation initiation factors eIF4E and eIFiso4E are required for polysome formation and regulate plant growth in tobacco.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 51 citations in OpenAlex.
- Imprecise Cas12a/ssODN-Mediated Editing of eIF4E1 Confers Dominant-Negative Resistance to Potato Virus Y in Solanum tuberosum.Molecular plant pathology · 2026Article
- Plant eIF4E isoforms as factors of susceptibility and resistance to potyviruses.Frontiers in plant science · 2023Review
- Effect of Gene Silencing of Translation Initiation Factors eIF(iso)4G and eIF(iso)4E on Sour Cherry Rootstock Resistance to Sharka Disease.International journal of molecular sciences · 2022Article
- CRISPR/Cas-Mediated Resistance against Viruses in Plants.International journal of molecular sciences · 2022Review
- CRISPR-Cas9 Targeting of theFrontiers in microbiology · 2022Article
- Mutation of barleyFrontiers in plant science · 2022Article
- eIF4E1 RegulatesFrontiers in plant science · 2022Article
- Loss-of-function ofBreeding science · 2021Article
- When a knockout is an Achilles' heel: Resistance to one potyvirus species triggers hypersusceptibility to another one in Arabidopsis thaliana.Molecular plant pathology · 2021Article
- Translation Initiation Factor eIF4E Positively Modulates Conidiogenesis, Appressorium Formation, Host Invasion and Stress Homeostasis in the Filamentous FungiFrontiers in plant science · 2021Article
- Soybean RNA interference lines silenced for eIF4E show broad potyvirus resistance.Molecular plant pathology · 2020Article
- A complex eIF4E locus impacts the durability of va resistance to Potato virus Y in tobacco.Molecular plant pathology · 2019Article
- Mutation of a Nicotiana tabacum L. eukaryotic translation-initiation factor gene reduces susceptibility to a resistance-breaking strain of Potato virus Y.Molecular plant pathology · 2018Article
- Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants.Plant biotechnology journal · 2018Article
- Translation Initiation Factor eIF4E and eIFiso4E Are Both Required forFrontiers in microbiology · 2017Article
- Variations of five eIF4E genes across cassava accessions exhibiting tolerant and susceptible responses to cassava brown streak disease.PloS one · 2017Article
- Plant Translation Factors and Virus Resistance.Viruses · 2015Review
- Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance.Molecular plant pathology · 2014Article
- Pyramiding resistances based on translation initiation factors in Arabidopsis is impaired by male gametophyte lethality.Plant signaling & behavior · 2014Article
- Silencing of the host factor eIF(iso)4E gene confers plum pox virus resistance in plum.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic initiation factor eIF4E plays a pivotal role in translation initiation. As a component of the ternary eIF4F complex, eIF4E interacts with the mRNA cap structure to facilitate recruitment of the 40S ribosomal subunit onto mRNA. Plants contain two distinct cap-binding proteins, eIF4E and eIFiso4E, that assemble into different eIF4F complexes. To study the functional roles of eIF4E and eIFiso4E in tobacco, we isolated two corresponding cDNAs, NteIF4E1 and NteIFiso4E1, and used these to deplete cap-binding protein levels in planta by antisense downregulation. Antibodies raised against recombinant NteIF4E1 detected three distinct cap-binding proteins in tobacco leaf extracts; NteIF4E and two isoforms of NteIFiso4E. The three cap-binding proteins were immuno-detected in all tissues analysed and were coordinately regulated, with peak expression in anthers and pollen. Transgenic tobacco plants showing significant depletion of either NteIF4E or the two NteIFiso4E isoforms displayed normal vegetative development and were fully fertile. Interestingly, NteIFiso4E depletion resulted in a compensatory increase in NteIF4E levels, whereas the down-regulation of NteIF4E did not trigger a reciprocal increase in NteIFiso4E levels. The antisense depletion of both NteIF4E and NteIFiso4E resulted in plants with a semi-dwarf phenotype and an overall reduction in polyribosome loading, demonstrating that both eIF4E and eIFiso4E support translation initiation in planta, which suggests their potential role in the regulation of plant growth.
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Registered trials
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