ArticlePlant molecular biology2005
Suppression of the cysteine protease, aleurain, delays floret senescence in Brassica oleracea.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 74 citations in OpenAlex.
- Cathepsin B- and L-like Protease Activities Are Induced During Developmental Barley Leaf Senescence.Plants (Basel, Switzerland) · 2024Article
- Regulation of senescence-associated protease genes by sulphur availability according to barley (Hordeum vulgare L.) phenological stage.Annals of botany · 2020Article
- Identification of candidate genes for leaf scorch in Populus deltoids by the whole genome resequencing analysis.Scientific reports · 2018Article
- Cell death patterns in Arabidopsis cells subjected to four physiological stressors indicate multiple signalling pathways and cell cycle phase specificity.Protoplasma · 2017Article
- Major Cys protease activities are not essential for senescence in individually darkened Arabidopsis leaves.BMC plant biology · 2017Article
- Subfamily-Specific Fluorescent Probes for Cysteine Proteases Display Dynamic Protease Activities during Seed Germination.Plant physiology · 2015Article
- TcCYPR04, a Cacao Papain-Like Cysteine-Protease Detected in Senescent and Necrotic Tissues Interacts with a Cystatin TcCYS4.PloS one · 2015Article
- Comprehensive analysis of cystatin family genes suggests their putative functions in sexual reproduction, embryogenesis, and seed formation.Journal of experimental botany · 2014Article
- Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana.Plant molecular biology · 2013Article
- Carbon deprivation-driven transcriptome reprogramming in detached developmentally arresting Arabidopsis inflorescences.Plant physiology · 2012Article
- The MADS29 transcription factor regulates the degradation of the nucellus and the nucellar projection during rice seed development.The Plant cell · 2012Article
- Efficient production of genetically engineered, male-sterile Arabidopsis thaliana using anther-specific promoters and genes derived from Brassica oleracea and B. rapa.Plant cell reports · 2008Article
- Identification of early senescence-associated genes in rice flag leaves.Plant molecular biology · 2008Article
- Identification of single nucleotide polymorphisms and analysis of linkage disequilibrium in sunflower elite inbred lines using the candidate gene approach.BMC plant biology · 2008Article
- Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers.Plant cell reports · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An aleurain-like protein, BoCP5, is up-regulated during harvest-induced senescence in broccoli floret and leaf tissue. BoCP5 is most closely related to an Arabidopsis protein (91%, AAF43041) and has 71% identity to barley aleurain (P05167). The mRNA for this gene accumulates within 6 h after harvest in broccoli florets, and its expression is reduced in tissue that has been held in senescence-delaying treatments (e.g. water, sucrose feeding, controlled atmosphere). The gene is also expressed in leaves during aging-related and harvest-induced senescence. Analysis of protein bands that cross-react with antibodies raised to the bacterial BoCP5 fusion protein, revealed prominent immunoreactive bands at ca. 26, 28, 31, and 38 kD in floret tissue. The 31 kD band was absent in protein extracts from leaf tissue. Agrobacterium-mediated transformation was used to produce transgenic broccoli plants with down-regulated BoCP5. A reduction in the postharvest expression of BoCP5 in floret tissue was achieved for four transgenic lines in the current study. In three of these lines postharvest floret senescence (yellowing) was delayed, and florets contained significantly greater chlorophyll levels during postharvest storage at 20 degrees C than wild-type plants. Line 4 showed the greatest down-regulation of BoCP5, and in this line postharvest protease activity remained at pre-harvest levels, and the yield of soluble proteins extracted from florets after harvest was significantly greater than that of wild-type tissue.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.