ArticlePlant molecular biology2005
Identification of cis-elements for ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Comprehensive analysis of IDD family genes and their expression patterns in barley.BMC plant biology · 2026Article
- The chloroplast redox-responsive transcriptome of solanaceous plants reveals significant nuclear gene regulatory motifs associated to stress acclimation.Plant molecular biology · 2022Article
- Cloning and functional characterization of seed-specific LEC1A promoter from peanut (Arachis hypogaea L.).PloS one · 2021Article
- Genome-wide analysis ofPeerJ · 2021Article
- Genome-wide identification and expression analysis of the phosphatase 2A family in rubber tree (Hevea brasiliensis).PloS one · 2020Article
- Ethylene Responsive Factor MeERF72 Negatively RegulatesInternational journal of molecular sciences · 2018Article
- Expression of the Intracellular COPT3-Mediated Cu Transport Is Temporally Regulated by the TCP16 Transcription Factor.Frontiers in plant science · 2018Article
- MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively RegulateFrontiers in plant science · 2017Article
- The Promoter of AtUSP Is Co-regulated by Phytohormones and Abiotic Stresses inFrontiers in plant science · 2016Article
- Characterization of the expression of the stress-responsivePlant biotechnology (Tokyo, Japan) · 2016Article
- De Novo Regulatory Motif Discovery Identifies Significant Motifs in Promoters of Five Classes of Plant Dehydrin Genes.PloS one · 2015Article
- Article
- Cloning and Characterization of 5' Flanking Regulatory Sequences of AhLEC1B Gene from Arachis Hypogaea L.PloS one · 2015Article
- Cloning and Expression Analysis of a Gene Encoding for Ascorbate Peroxidase and Responsive to Salt Stress in Beet (Plant molecular biology reporter · 2014Article
- The gene encoding Arabidopsis acyl-CoA-binding protein 3 is pathogen inducible and subject to circadian regulation.Journal of experimental botany · 2012Article
- Regulation of the fruit-specific PEP carboxylase SlPPC2 promoter at early stages of tomato fruit development.PloS one · 2012Article
- Characterization of a F-box gene up-regulated by phytohormones and upon biotic and abiotic stresses in grapevine.Molecular biology reports · 2011Article
- Tomato polyphenol oxidase B is spatially and temporally regulated during development and in response to ethylene.Molecules (Basel, Switzerland) · 2011Article
- Molecular cloning and function assay of a chalcone isomerase gene (GbCHI) from Ginkgo biloba.Plant cell reports · 2011Article
- Analysis of a new strain of Euphorbia mosaic virus with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region.Virology journal · 2010Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions and by in vitro binding assays. Analysis of transgenic tobacco transformed with SmCP promoter-GUS constructs confirmed that the promoter region -415/+54 containing Ethylene Responsive Element ERE(-355/-348) conferred threefold ethylene-induction of GUS expression, while -827/+54 which also contains ERE(-683/-676), produced fivefold induction. Using gel mobility shift assays, we demonstrated that each ERE binds nuclear proteins from both ethephon-treated and untreated 5-week-old seedlings, suggesting that different transcriptions factors bind each ERE under varying physiological conditions. Binding was also observed in extracts from senescent, but not young, fruits. The variation in binding at the EREs in fruits and seedlings imply that organ-specific factors may participate in binding. Analysis of transgenic tobacco expressing various SmCP promoter-luc constructs containing wild-type or mutant Evening Elements (EEs) confirmed that both conserved EEs at -795/-787 and -785/-777 are important in circadian control. We confirmed the binding of total nuclear proteins to EEs in gel mobility shift assays and in DNase I footprinting. Our results suggest that multiple proteins bind the EEs which are conserved in plants other than Arabidopsis and that functional EEs and EREs are present in the 5'-flanking region of a gene encoding cysteine proteinase.
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