ArticleMolecular biotechnology2005
Expression of foreign genes in Dunaliella by electroporation.
Article in Molecular biotechnology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity.Biotechnology for biofuels and bioproducts · 2024Article
- Gene transfer to plants by electroporation: methods and applications.Molecular biology reports · 2020Review
- Variation of fatty acid desaturation in response to different nitrate levels inRoyal Society open science · 2018Article
- Anti-HBV effect of interferon-thymosin α1 recombinant proteins in transgenicExperimental and therapeutic medicine · 2018Article
- Genetic Engineering ofFrontiers in microbiology · 2018Article
- Development of an alcohol-inducible gene expression system for recombinant protein expression inJournal of applied phycology · 2018Article
- Efficient and stable transformation ofBioImpacts : BI · 2017Article
- Current status and perspectives of genome editing technology for microalgae.Biotechnology for biofuels · 2017Review
- Metabolic engineering of Dunaliella salina for production of ketocarotenoids.Photosynthesis research · 2016Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Article
- The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins.World journal of microbiology & biotechnology · 2014Review
- Hypoosmotic expression of Dunaliella bardawil ζ-carotene desaturase is attributed to a hypoosmolarity-responsive element different from other key carotenogenic genes.Plant physiology · 2014Article
- Article
- Promoter trapping in microalgae using the antibiotic paromomycin as selective agent.Marine drugs · 2012Article
- A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina.Current microbiology · 2012Article
- Sequence analysis and potentials of the native RbcS promoter in the development of an alternative eukaryotic expression system using green Microalga Ankistrodesmus convolutus.International journal of molecular sciences · 2012Article
- The cloning and characterization of two ammonium transporters in the salt-resistant green alga, Dunaliella viridis.Molecular biology reports · 2011Article
- The characterization of two peroxiredoxin genes in Dunaliella viridis provides insights into antioxidative response to salt stress.Plant cell reports · 2011Article
- Molecular cloning and characterization of a vacuolar H+₋pyrophosphatase from Dunaliella viridis.Molecular biology reports · 2011Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An electroporation procedure has been described for introducing plasmid DNA into Dunaliella salina cells. By this procedure, a bulk of plasmid DNA was delivered into the cells and retained for at least 3 d. Reverse transcriptase polymerase chain reaction (RT-PCR) and sequencing analyses indicated that the transcription and pre-mRNA splicing of ble gene (contributing the Zeocin resistance) were detected in the cells as early as 1 h after the electroporation. Individual colonies could retain the resistance to 10 mg/L Zeocin for at least 6 mo. Subsequent Southern blot analysis showed the existence of introduced plasmid DNA inside these colonies. However, most of the cells (approx 90%) lost the resistance in the presence of 5 mg/L Zeocin during subculturing, which was consistent with the observations of both rearranged and episomal plasmid DNA existed in the cells. Nevertheless, the electroporation procedure allows introducing a gene of interest and studying its expression and function in D. salina cells.
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