ArticleMolecular and cellular biology2005
Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 100 papers.
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100 citing papers in PubMed.
- Rapid protamine evolution suppresses meiotic drive inScience advances · 2026Article
- Counteraction of HMGB1 at ss-dsDNA junctions maintains liquidity of protamine-DNA co-condensates.Nature communications · 2026Article
- The jewel wasp Nasonia vitripennis utilizes two single-copy, protamine-like sperm nuclear basic proteins.G3 (Bethesda, Md.) · 2026Article
- The Nuclear Pore Complex Facilitates Centriole-Nuclear Attachment in Spermatids.bioRxiv : the preprint server for biology · 2026Article
- Counteraction of HMGB1 at ss-dsDNA junctions maintains liquidity of protamine-DNA co-condensates.bioRxiv : the preprint server for biology · 2026Article
- Selfish chromosomes exploit a germline checkpoint to eliminate competing gametes.Nature communications · 2026Article
- A comparison of spermatogenesis between flies and men-conserved processes of male gamete production.Human reproduction update · 2025Review
- The physical consequences of sperm gigantism.bioRxiv : the preprint server for biology · 2025Article
- Intrinsically weak sex chromosome drive through sequential asymmetric meiosis.Science advances · 2025Article
- Testis-specific serine/threonine kinase dTSSK2 regulates sperm motility and male fertility in Drosophila.Communications biology · 2025Article
- Cytoplasmic incompatibility factor proteins fromProceedings. Biological sciences · 2025Article
- Running the gauntlet: challenges to genome integrity in spermiogenesis.Nucleus (Austin, Tex.) · 2024Review
- The protamines of the spider Steatoda sp. provide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis.Development (Cambridge, England) · 2024Article
- Article
- The protamines of the noble false widow spiderbioRxiv : the preprint server for biology · 2024Article
- Protamines and the sperm nuclear basic proteins Pandora's Box of insects.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2024Article
- Article
- Metabolic regulation of proteome stability via N-terminal acetylation controls male germline stem cell differentiation and reproduction.Nature communications · 2023Article
- Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome.Communications biology · 2023Article
- Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster.Scientific reports · 2023Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin condensation is a typical feature of sperm cells. During mammalian spermiogenesis, histones are first replaced by transition proteins and then by protamines, while little is known for Drosophila melanogaster. Here we characterize three genes in the fly genome, Mst35Ba, Mst35Bb, and Mst77F. The results indicate that Mst35Ba and Mst35Bb encode dProtA and dProtB, respectively. These are considerably larger than mammalian protamines, but, as in mammals, both protamines contain typical cysteine/arginine clusters. Mst77F encodes a linker histone-like protein showing significant similarity to mammalian HILS1 protein. ProtamineA-enhanced green fluorescent protein (eGFP), ProtamineB-eGFP, and Mst77F-eGFP carrying Drosophila lines show that these proteins become the important chromosomal protein components of elongating spermatids, and His2AvDGFP vanishes. Mst77F mutants [ms(3)nc3] are characterized by small round nuclei and are sterile as males. These data suggest the major features of chromatin condensation in Drosophila spermatogenesis correspond to those in mammals. During early fertilization steps, the paternal pronucleus still contains protamines and Mst77F but regains a nucleosomal conformation before zygote formation. In eggs laid by sesame-deficient females, the paternal pronucleus remains in a protamine-based chromatin status but Mst77F-eGFP is removed, suggesting that the sesame gene product is essential for removal of protamines while Mst77F removal is independent of Sesame.
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